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Presto™ 96 well Viral Nucleic Acid Extraction Kit (96VR02, 96VR04, 96VR10)

96 Well DNA/RNA Extraction/96 Well Virus Extraction kit

The Presto™ 96 well Viral Nucleic Acid Extraction Kit was designed for high-throughput purification of high-quality of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. Detergents and chaotropic salt are used to lyse and inactivate viruses. The efficient glass fiber in each well is optimized for nucleic acid purification from a wide variety of both DNA and RNA viruses such as HBV, CMV, HCV, HIV, and HTLV. 10E1-10E9 copies of viral DNA/RNA can be purified from 200 µl of serum within 60 minutes. The purified viral DNA/RNA can be used directly in qPCR and qRT-PCR assays.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # 96VR02, 96VR04, 96VR10)

  • High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number!
  • Purify virus DNA or virus RNA from 96 samples simultaneously in 60 minutes.
  • Sample Volume: up to 200 µl samples of plasma, serum, body fluids, supernatant of viral cell cultures
  • Viral DNA/RNA 96 Well Binding Plate: glass fiber membrane optimized for virus DNA and virus RNA purification
  • Viral DNA/RNA 96 Well Binding Plates were certified RNase and DNase-free
  • Elution Volume: 50-80 µl
  • Storage: dry at room temperature (15-25ºC)

APPLICATIONS

RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS)

COMPONENTS

  • VB Lysis Buffer
  • AD Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • Carrier RNA
  • Presto™ Viral DNA/RNA 96 Well Binding Plate
  • 96 Deep Well Plate
  • 0.35 ml Collection Plates

QUALITY CONTROL

The quality of PrestoTM 96 well Viral Nucleic Acid Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample.

Viral Nucleic Acid Extraction Kit II Functional Test Data

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Figure 1 .Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the PrestoTM 96 well Viral Nucleic Acid Extraction Kit (3 replications of each copy number). The purified RNA was eluted with 50 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 3 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV. The average cycle threshold (Ct): 10E4 = 23.88, 10E3 = 27.72, 10E2 = 31.22, 10E1 = 34.62. The low Ct values indicate a high number of target nucleic acid in the sample.

 

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