旭基科技股份有限公司

Advantages (Cat. # DRP050, DRP100)

• Purify genomic DNA, total RNA within 25 minutes! Protein precipitation within 50 minutes!
• Variety of Samples: up to 500 μl of whole blood, up to 25 mg of tissue, rodent tails, ear punches, up to 5 x 10⁶ cultured cells, up to 200 μl of biological fluids such as serum and plasma
• High Yield: up to 9 μg of genomic DNA, up to 20 μg of total RNA from 1.5 x 10⁶ of cultured HeLa cells, up to 1 μg of genomic DNA, up to 10 μg of total RNA from 500 µl human blood samples
• High Quality DNA: A260/A280 = 1.9-2.0
• Genomic DNA glass fiber spin columns and total RNA glass fiber spin colums certified DNase and RNase-free
• Elution Volume: 50-200 μl (genomic DNA) and 25-50 μl (total RNA)
• Storage: dry at room temperature (15-25°C)

APPLICATIONS

The purified DNA, approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-time PCR, northern blotting, primer extension, mRNA selection and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.

COMPONENTS

• RBC Lysis Buffer
• DR Buffer
• RW1 Buffer
• RPE Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
• DV Buffer (8M Urea)
• Protein Loading Dye
• GD Columns
• RB Columns
• 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Presto™ DNA/RNA/Protein Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA, total RNA and total protein from 1.5 x 10⁶ cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. The purified protein is quantified by bradford assay and analyzed on SDS-PAGE.

Presto™ DNA RNA Extraction Kit Functional Test Data

Figure 1 .Genomic DNA and Total RNA from 1.5 x 10⁶ HeLa cells was extracted using the Presto™ DNA/RNA/Protein Extraction Kit. 10 µl aliquots from 200 µl eluates of purified genomic DNA and 10 µl aliquots from 50 µl eluates of purified total RNA was analyzed by electrophoresis on a 1% agarose gel.

1-2 = DNA from 1.5 x 10⁶ HeLa cells
3-4 = RNA from 1.5 x 10⁶ HeLa cells
M = Geneaid 1 Kb DNA Ladder

 

Sample

µg/ml

260/280

260/280

Yield

1. DNA

42.7

1.88

2.19

8.54 µg

2. DNA

46.7

1.88

2.18

9.34 µg

3. RNA

417.5

2.09

2.03

20.9 µg

4. RNA

429.9

2.09

2.04

21.5 µg