旭基科技股份有限公司

Advantages (Cat. # PVR050, PVR100)

• Purify high-quality virus RNA from a variety of plant species (including those with high levels of polysaccharide inhibitors)
• Sample Volume: up to 100 mg of fresh or frozen plant tissue
• Elution Volume: 50 µl
• Spin Columns: glass fiber membrane optimized for virus RNA purification
• Individually packaged virus spin columns and collection tubes, certified RNase and DNase-free
• Storage: dry at room temperature (15-25ºC) for up to 9 months without showing any reduction in performance

APPLICATIONS

RT-PCR, RT-qPCR, Next Generation Sequencing (NGS), Northern Blotting

COMPONENTS

• PVR Buffer
• PVRS Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• PV Columns
• 2 ml Collection Tubes

QUALITY CONTROL

The Plant Virus RNA Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Virus RNA is purified from leaf discs of grapevine plants infected with multiple viruses. Following RNA purification a 5 µl aliquot from a 50 µl eluate of purified viral RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Plant Virus RNA Kit Real-Time PCR Test Data

Figure 1 .Real-Time PCR data confirms GLRaV-3 was successfully detected and identified following virus RNA extraction using the Geneaid Plant Virus RNA Kit. Increased analytical sensitivity was evident by the earlier (lower) Cq – Cycle quantity – value compared to Competitor Q.