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96 Well Tissue DNA Kit (96GS004, 96GS010)

96 Well DNA/RNA Extraction/96 Well DNA Extraction

The 96 Well gSYNC™ DNA Extraction Kit is optimized for high-throughput genomic DNA extraction from tissue and cells. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the 96 Well Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 60 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # 96GS004, 96GS010)

  • Purify 96 wells of genomic DNA within 1 hour!
  • Variety of Samples: tissue, rodent tails, ear punches and cultured cells
  • High Yield: 10-20 µg from 20 mg of tissue
  • High Quality DNA: A260/A280 = 1.8-2.0
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 100-400 µl
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

  • GST Buffer
  • GSB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Proteinase K
  • RNase A
  • 96 Well gDNA Binding Plates
  • Microtubes (Racked)
  • Microtubes (8-strip)
  • Caps for Microtubes (8-strip)
  • 96 Deep Well Plates

QUALITY CONTROL

The quality of the 96 Well gSYNC™ DNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 200 μl of whole human blood is extracted using the gSYNC™ DNA Extraction Kit. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

gSYNC™ DNA Extraction Kit Functional Test Data

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Figure 1 .Genomic DNA from 10 mg mouse tissue was extracted using the 96 Well gSYNC™ DNA Extraction Kit. 5 µl aliquots from a 200 µl eluate of purified genomic DNA were analyzed by electrophoresis on a 0.8% agarose gel.

1-3 = kidney, 4-6 = muscle, 7-9 = liver, 10-12 = spleen
M = Geneaid 1 Kb DNA Ladder

 
 
Sample (Mouse)
Yield
260/280
260/230
1
Kidney (10 mg)
7.4 µg
1.81
2.03
2
Kidney (10 mg)
8.0 µg
1.82
2.06
3
Kidney (10 mg)
8.6 µg
1.82
2.07
4
Muscle (10 mg)
3.0 µg
1.88
1.86
5
Muscle (10 mg)
4.2 µg
1.89
1.79
6
Muscle (10 mg)
3.4 µg
1.90
1.73
7
Liver (10 mg)
7.2 µg
1.81
2.03
8
Liver (10 mg)
7.1 µg
1.83
1.98
9
Liver (10 mg)
7.3 µg
7.78
1.97
10
Spleen (10 mg)
13.0 µg
1.83
2.22
11
Spleen (10 mg)
12.7 µg
1.83
2.16
12
Spleen (10 mg)
12.0 µg
1.81
2.03

Plasmid DNA Midi and Maxi Kits Specifications

 
SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnReagent SystemSpin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg50 µg (Mini)
500 µg (Maxi)
50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
 25 mg animal tissue200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
 1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
 hair2x108 yeast/fungus    1-100 µl saliva 
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme Proteinase KLyticase or ZymolaseProteinase KProteinase K
Typical Yield5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10-15 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
 10-40 µg from
20 mg tissue
  80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109Bacillus subtilis   
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)
30-200 µl50-100 µl30-100 µl50-100 µl
Operation Time<20 minutes<25 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes

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