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Magnetic Beads gDNA Kit (Food) MF048/MF096

Magnetic Beads DNA/RNA Extraction/Magnetic Beads gDNA kit Food

The Magnetic Beads Genomic DNA Extraction Kit (Food) is designed for rapid isolation of DNA from a variety of raw and processed food samples. The unique MF1 Buffer when combined with Proteinase K is used to efficiently digest food tissue and proteins. PCR inhibitors such as polysaccharides and plant metabolites are removed by centrifugation. Chloroform treatment, separates residual inhibitors from the clear supernatant in the organic phase. DNA in the aqueous phase is then bound by the MF Magnetic Beads followed by wash and elution. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

DOCUMENTS

DESCRIPTION

Advantages (Cat. # MF048/MF096)

  • High Quality DNA: >A260/A280 = 1.8-2.0
  • Sample: up to 100 mg of raw or processed food samples
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Sample: up to 100 µl of PCR products
  • Manual or automated DNA isolation
  • Operation time: within 50 minutes (manual) 
  • Storage: dry at room temperature (15-25ºC)

APPLICATIONS

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

COMPONENTS

  • MF1, MF2 Buffer
  • Proteinase K
  • MW1, MW2 Buffer
  • MF Magnetic Beads
  • Elution Buffer

QUALITY CONTROL

The quality of the The Magnetic Beads gDNA Kit (Food) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA is purified from 100 mg food samples and analyzed by electrophoresis.

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