旭基科技股份有限公司

Specifications (Cat. # 96VR02, 96VR04, 96VR10)

• High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number!
• Purify virus DNA or virus RNA from 96 samples simultaneously in 60 minutes.
• Sample Volume: up to 200 µl samples of plasma, serum, body fluids, supernatant of viral cell cultures
• Viral DNA/RNA 96 Well Binding Plate: glass fiber membrane optimized for virus DNA and virus RNA purification
• Viral DNA/RNA 96 Well Binding Plates were certified RNase and DNase-free
• Elution Volume: 50-80 µl
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS

RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS)

COMPONENTS

• VB Lysis Buffer
• AD Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• Carrier RNA
• Presto™ Viral DNA/RNA 96 Well Binding Plate
• 96 Deep Well Plate
• 0.35 ml Collection Plates

QUALITY CONTROL

The quality of PrestoTM 96 well Viral Nucleic Acid Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample.

Viral Nucleic Acid Extraction Kit II Functional Test Data

Figure 1 .Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the PrestoTM 96 well Viral Nucleic Acid Extraction Kit (3 replications of each copy number). The purified RNA was eluted with 50 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 3 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV. The average cycle threshold (Ct): 10E4 = 23.88, 10E3 = 27.72, 10E2 = 31.22, 10E1 = 34.62. The low Ct values indicate a high number of target nucleic acid in the sample.