旭基科技股份有限公司

Advantages (Cat. # TRPD050, TRPD100, TRPD200)

• Purify total RNA in 10 minutes!
• No chlorophorm phase separation
• No isopropanol RNA precipitation
• No phenol carryover
• Compatibility: homogenize samples with GENEzol™, TRI-Reagent®, TRIzol®, RNAzol®, QIAzol® etc.
• Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma), up to 5 x 10⁶ cultured cells, 10-50 mg of tissue, 1 x 10⁹ bacterial cells, 20-50 mg of plant tissue
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
• Membrane: glass fiber membrane optimized for total RNA extraction
• Spin Columns: individually packaged RNA spin columns, certified RNase and DNase-free
• Storage: dry at room temperature (15-25ºC), DNase I can be shipped at room temperature but should be stored at -20ºC for extended periods

APPLICATIONS

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

COMPONENTS

• Pre-Wash Buffer
• DNase I
• DNase I Reaction Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

QUALITY CONTROL

The TriRNA Pure Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. An Escherichia coli (1 × 10⁹) culture (OD600=1.3, 1 ml) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by GENEzol™ Reagent homogenization. RNA is then purified using a spin column procedure. 10 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

TriRNA Pure Kit Functional Test Data

Figure 1 .RNA was purified using the TriRNA Pure Kit in parallel to the similar product from competitor Z. 5x10⁵ HeLa cells were homogenized using GENEzol™ Reagent and competitor Z tri reagent. RNA was then purified using the corresponing kits spin column procedure. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.

 

Test

RNA Conc.

260/280

260/230

Yield

1. Z

162.5 ng/µl

2.00

2.07

8.1 µg

2. Z

160.7 ng/µl

2.03

2.07

8.0 µg

3. Geneaid

164.0 ng/µl

2.00

2.07

8.2 µg

4. Geneaid

161.6 ng/µl

2.03

2.06

8.0 µg

Table 1 .Total RNA purified using the TriRNA Pure Kit and competitor Z.