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GENEzol™ TriRNA Pure Kit (GZX050/GZX100/GZX200) without DNase

RNA Extraction/Tri RNA

The GENEzol™ TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

DOCUMENTS

DESCRIPTION

Advantages (Cat. # GZX050, GZX100, GZX200)

  • Purify total RNA in 15 minutes!
  • A cost effective phenol, guanidine isothiocyanate solution plus spin column system
  • No chlorophorm phase separation
  • No isopropanol RNA precipitation
  • No phenol carryover
  • Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue
  • High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  • Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
  • Spin Columns: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
  • Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, other components should be stored at room temperature (15-25ºC)

APPLICATIONS

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

COMPONENTS

  • GENEzol™ Reagent
  • Pre-Wash Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The GENEzol™ TriRNA Pure Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system.

GENEzol™ TriRNA Pure Kit Interactive Protocol

GENEzol™ TriRNA Pure Kit Functional Test Data

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Figure 1 .RNA was purified using the GENEzol™ TriRNA Pure Kit in parallel to the similar product from competitor Z. 5x105 HeLa cells were homogenized using GENEzol™ Reagent and competitor Z tri reagent. RNA was then purified using the corresponing kits spin column procedure. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.

 
Test
RNA Conc.
260/280
260/230
Yield
1. Z
162.5 ng/µl
2.00
2.07
8.1 µg
2. Z
160.7 ng/µl
2.03
2.07
8.0 µg
3. Geneaid
164.0 ng/µl
2.00
2.07
8.2 µg
4. Geneaid
161.6 ng/µl
2.03
2.06
8.0 µg

Table 1 .Total RNA purified using the GENEzol™ TriRNA Pure Kit and competitor Z.

 
Test
RNA Conc.
260/280
260/230
Yield
1
58.6 ng/µl
1.86
1.81
2.1 µg
2
68.1 ng/µl
1.87
1.81
2.4 µg
3
74.2 ng/µl
1.87
1.83
2.6 µg

Table 2 .RNA was purified using the GENEzol™ TriRNA Pure Kit. RNA from 200 µl of whole human blood (3 donors) was initally homogenized using GENEzol™ Reagent. RNA was then purified using a spin column procedure. 10 µl from each 35 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel.

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RNA was extracted from 200 µl of human blood (5.2 x 1010 copy/ml) using GENEzol™ TriRNA Pure Kit (red line).
RNA standard (107 copy/ml) (light green line)
RNA standard (106 copy/ml) (dark green line)
RNA standard (105 copy/ml) (light blue line)
RNA standard (104 copy/ml) (dark blue line)

Figure 3 .Quantitative analysis of human beta globin mRNA purified by GENEzol™ TriRNA Pure Kit using a Taqman probe 1-step qRT-PCR assay. The assay was run on a BioRad IQ5 thermal cycler.

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