旭基科技股份有限公司

Advantages (Cat. # GZBD050, GZBD100, GZBD200)

• Purify total RNA within 20 minutes without chloroform phase separation or isopropanol RNA precipitation
• Sample Volume: bacteria cells up to 1 x 10⁹
• Format: phenol, chlorform, guanidine isothiocyanate plus spin column system
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, DNase I and Lysozyme can be shipped at room temperature but should be stored at -20ºC for extended periods

APPLICATIONS

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

COMPONENTS

• GENEzol™ Reagent
• Pre-Wash Buffer
• Lysozyme
• Bacteria Lysis Buffer
• DNase I
• DNase Reaction Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

QUALITY CONTROL

The GENEzol™ TriRNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 5 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 1% agarose gel.

GENEzol™ TriRNA Bacteria Kit Functional Test Data

Figure 1 .RNA was extracted using the GENEzol™ TriRNA Bacteria Kit. An Escherichia coli (1×10⁹) culture (OD600=2, 1 ml) was harvested by centrifugation at 16,000 x g for 2 minutes, followed by RNA extraction. 5 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 

Test

RNA Concentration

260/280

260/230

Yield

1

502.6 µg/ml

2.06

2.18

25.13 µg

2

518.3 µg/ml

2.07

2.21

25.92 µg

3

506.0 µg/ml

2.08

2.24

25.30 µg