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GENEzol™ Bacteria Kit (GZB050/GZB100/GZB200)

RNA Extraction/Tri RNA

The GENEzol™ TriRNA Bacteria Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from bacteria samples. Bacterial cell walls are initially lysed using Lysozyme. The sample is then homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

DOCUMENTS

DESCRIPTION

Advantages (Cat. # GZB050, GZB100, GZB200)

  • Purify total RNA within 20 minutes without chloroform phase separation or isopropanol RNA precipitation
  • Sample Volume: bacteria cells up to 1 x 109
  • Format: phenol, chlorform, guanidine isothiocyanate plus spin column system
  • High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  • Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, Lysozyme can be shipped at room temperature but should be stored at -20ºC for extended periods

APPLICATIONS

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

COMPONENTS

  • GENEzol™ Reagent
  • Pre-Wash Buffer
  • Lysozyme
  • Bacteria Lysis Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The GENEzol™ TriRNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 5 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 1% agarose gel.

GENEzol™ TriRNA Bacteria Kit Functional Test Data

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Figure 1 .RNA was extracted using the GENEzol™ TriRNA Bacteria Kit. An Escherichia coli (1×109) culture (OD600=2, 1 ml) was harvested by centrifugation at 16,000 x g for 2 minutes, followed by RNA extraction. 5 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 
Test
RNA Concentration
260/280
260/230
Yield
1
502.6 µg/ml
2.06
2.18
25.13 µg
2
518.3 µg/ml
2.07
2.21
25.92 µg
3
506.0 µg/ml
2.08
2.24
25.30 µg

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