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Plant Total RNA Mini Kit (RP050, RP100, RP300) without DNase

RNA Extraction/Total RNA

The Total RNA Mini Kit (Plant) is a RNA Isolation Kit designed for total RNA purification from plant tissue and cells using an efficient RNA miniprep system. This RNA Kit will purify RNA from a wide variety of plant species within 15 minutes. Samples are initially ground in liquid nitrogen and then filtered to remove cell debris using the efficient RNA filter columns. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the RNA spin column. Once any contaminants have been removed using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified total RNA is ready for use in RT-PCR, Real-time PCR and Northern Blotting. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # RP050, RP100, RP300)

  • Purify total RNA within 15 minutes!
  • High Yield: 5-30 μg of pure RNA
  • Sample Size: up to 100 mg (fresh plant tissue), up to 25 mg (dry plant tissue)
  • Spin Columns: glass fiber membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

RT-PCR, Northern Blotting, Real-time PCR

COMPONENTS

  • RB Buffer
  • PRB Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • Filter Columns
  • RB Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Total RNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from a 50 mg fresh Thale Cress (Arabidopsis thaliana) leaves is extracted using the Total RNA Mini Kit (Plant). The purified RNA (27 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Total RNA Mini Kit (Plant) Functional Test Data

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Figure 1 .Total RNA from 50 mg Thale Cress (Arabidopsis thaliana) leaves was extracted using the Total RNA Mini Kit (Plant). The purified RNA was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 
Test
Yield (μg)
260/280
260/230
Test 1
27.02
2.07
2.43
Test 2
27.68
2.10
2.40
Test 3
26.15
2.06
2.44
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Figure 2 .50 mg of Arabidopsis leaves RNA was purified using Geneaid Total RNA Mini Kit (Plant) Kit. The integrity and quality of RNA were determined by Qubit with RNA integrity and quality (IQ) assay kit. The IQ 8.8 score showed 88% large and structured RNA (including mRNA, tRNA and rRNA) was in the purified RNA.

 

 

Total RNA Extraction Kits Specifications

 
SpecificationsTotal RNA Mini Kit Blood Cultured CellTotal RNA Mini Kit TissueTotal RNA Mini Kit PlantPresto™ Mini RNA Bacteria KitPresto™ Mini RNA Yeast KitmiRNA Isolation KitGENEzol™ TriRNA Pure Kit
Cat. #RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050GZX050
GZX100
GZX200
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnTri Reagent Spin Column
Binding Capacity50 µg50 µg50 µg50 µg50 µg50 µg50 µg
Sampleblood, cellsanimal tissueplant tissuebacterial cellsyeast cellsanimal tissue, cellsblood,cells,
tissue, body fluids, bacteria
MicropestleN/AYesN/AN/AN/AYesN/A
Filter ColumnN/AYesYesN/AN/AN/AN/A
EnzymeN/AN/AN/ALysozymeN/AN/AN/A
Typical Yield2-3 µg from 300 µl blood, 25-30 µg from 106 cells5-30 µg5-30 µg40-50 µg from 1x109 bacterial cells15-20 µg from 5x107 yeast cells5-30 µg5-30 µg
Elution Volume50 µl50 µl50 µl50 µl50 µl50 µl25-50 µl
Time<20 min.<15 min.<20 min.<20 min.<20 min.<30 min.<15 min.

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