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Presto™ miRNA Purification Kit (PMI004, PMI050, PMI100)

RNA Extraction/Total RNA

The Presto™ miRNA Purification Kit is designed for purifying and enriching of small RNA molecules (< 200 nt) from a wide variety of samples, such as animal tissues, cultured cells and bacterial cells. These small RNAs include microRNA (miRNA), short interfering RNA (siRNA), as well as tRNA and 5S ribosomal RNA. miRNAs and siRNAs are typically 20-25 nucleotides long and vital for regulating gene expression by binding to mRNA molecules and affecting their stability or translation. Purification is based on spin column technology without the use of phenol and chloroform. Briefly, the cells or tissues are lysed using detergents and chaotropic salt. The larger RNA fragments in the chaotropic salt are bound by the glass fiber matrix of the first spin column while the smaller RNA molecules pass through into the flow-through. The smaller RNA molecules in the flow-through are bound by the second spin column once any contaminants have been removed using the Wash Buffer. The small RNA molecules are then eluted using the RNase-free water, and are ready to be used in various applications, including RT-PCR, northern blotting and microarray analysis.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # PMI004, PMI050, PMI100)

  • Purify small RNA molecules (< 200 nt) within 30 minutes!
  • Spin Columns: glass fiber membrane optimized for micro RNA extraction
  • No phenol or chloroform extractions
  • Minimal contamination from large RNA fragments
  • Large RNA fragments can be separately isolated during the extraction procedure
  • High quality small RNA can be used in various downstream applications
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

RT-PCR, Northern Blotting, microarray analysis

COMPONENTS

  • PMI Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the  Presto™ miRNA Purification Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Small RNAs from 1 x 106 Hela cells  are extracted using the Presto™ miRNA Purification Kit. The purified small RNA  is analyzed using capillary electrophoresis.

Presto™ miRNA Purification Kit Functional Test Data

Figure 1. Efficient Removal of Large RNAs and Enrichment of Small RNAs. Different RNA species were isolated from E. coli using Geneaid PrestoTM miRNA Purification Kit, and the purified RNAs were analyzed by capillary electrophoresis (BIOptic Qsep100). Panel (A) shows the large RNA fragments were removed by the first RNA columns. Panel (B) shows the small RNA molecules that were isolated and enriched using the second RNA columns and shows that there is no contamination with any large RNA fragments. This demonstrates the effective separation of the small RNA from the large RNA species using Geneaid PrestoTM miRNA Purification Kit.

 

 

Total RNA Extraction Kits Specifications

 
SpecificationsTotal RNA Mini Kit Blood Cultured CellTotal RNA Mini Kit TissueTotal RNA Mini Kit PlantPresto™ Mini RNA Bacteria KitPresto™ Mini RNA Yeast KitmiRNA Isolation KitGENEzol™ TriRNA Pure Kit
Cat. #RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050GZX050
GZX100
GZX200
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnTri Reagent Spin Column
Binding Capacity50 µg50 µg50 µg50 µg50 µg50 µg50 µg
Sampleblood, cellsanimal tissueplant tissuebacterial cellsyeast cellsanimal tissue, cellsblood,cells,
tissue, body fluids, bacteria
MicropestleN/AYesN/AN/AN/AYesN/A
Filter ColumnN/AYesYesN/AN/AN/AN/A
Enzyme>/th>N/AN/AN/ALysozymeN/AN/AN/A
Typical Yield2-3 µg from 300 µl blood, 25-30 µg from 106 cells5-30 µg5-30 µg40-50 µg from 1x109 bacterial cells15-20 µg from 5x107 yeast cells5-30 µg5-30 µg
Elution Volume50 µl50 µl50 µl50 µl50 µl50 µl25-50 µl
Time<20 min.<15 min.<20 min.<20 min.<20 min.<30 min.<15 min.

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