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96 Well G-50 Gel Filtration Plate (CGP04, CGP10)

DNA Extraction/Sequencing Cleanup

96-Well G-50 Gel Filtration Plates are pre-packed with a highly efficient gel filter which is ideal for removing excess unincorporated dye terminators, freeing nucleotides from sequencing and labeling reactions, protein purification, desalting and buffer exchange. Low molecular weight material is retained in the gel matrix, facilitating the purification of DNA fragments larger than 20 bases in length. Since 96-Well G-50 Gel Filtration Plates are designed to purify DNA fragments >20 bases only, they are not recommended for PCR product primer removal.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # CGP04, CGP10)

  • Purification in only 10 minutes!
  • Sample Volume: 20 to 50 μl
  • Filtrate: > 20 bases
  • Storage: 2-8ºC for up to 6 months (Do not freeze)

APPLICATIONS

  • Removing excess unincorporated dye terminators
  • Freeing nucleotides from sequencing and labeling reactions
  • Protein purification
  • Desalting
  • Buffer exchange

COMPONENT

96-Well G-50 Gel Filtration Plates are pre-packed with a highly efficient gel filter

QUALITY CONTROL

The quality of the 96-Well G-50 Gel Filtration Plate is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system.

 

G-50 Gel Filtration Dye Terminator Removal Column Protocol Procedure

 
Sample
DNA Concentration
Total DNA
Recovery
Original DNA
23.70 ng/µl
1185 ng
----------
Test 1
18.70 ng/µl
935 ng
78.90%
Test 2
18.20 ng/µl
910 ng
76.80%

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