旭基科技股份有限公司

Fecal samples were preserved using SYNCstore^TM STM Fecal Collection Tubes and the Z brand Fecal Collection Kit, each stored for 7, 14, and 28 days. After nucleic acid purification from the preserved samples, bacterial composition analysis was performed using Nanopore sequencing technology. The results showed that fecal samples stored in SYNCstore^TM STM Fecal Collection Tubes for 7, 14, and 28 days exhibited highly similar microbial profiles (Figure 1). In the Principal Coordinate Analysis (PCoA) plot, samples preserved with SYNCstore^TM STM clustered closely together, indicating minimal variation in microbial composition under these storage conditions and demonstrating excellent stability (Figure 2). The performance of the Geneaid product was superior to that of the Z brand.

Additional Influenza A Virus Added to SYNCstore^TM STM Fecal Collection Tube, Stored at 25°C for 1, 7, and 14 Days
Influenza A virus was added to fecal sample storage in SYNCstore^TM STM Fecal Collection Tube and stored at 25°C for 1, 7, and 14 days. Influenza A virus RNA was purified from the preserver and subjected to RT-qPCR and RT-PCR analysis. The qPCR results showed that the Ct values for influenza A virus in the samples stored for 1, 7, and 14 days were nearly identical, indicating that the influenza A virus RNA remains stable for up to 14 days (Figure 3). RT-PCR results demonstrated that after 14 days of storage, all gene fragments of the influenza A virus could still be successfully amplified, suggesting that the virus RNA maintains high integrity even after 14 days of storage in the Fecal Collection Tube (Figure 4).