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Plant DNA Isolation Kit (GR100, GR200)

Reagent DNA Extraction/Reagent DNA Extraction

GENEzol™ DNA Reagent Plant provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP without phenol extraction.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # GR100, GR200)

  • Extract high molecular weight genomic DNA from a variety of plant species
  • Sample Volume: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
  • A cost effective, scalable solution
  • Format: simple and gentle CTAB and chloroform based DNA precipitation method
  • Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase should be stored at 4ºC for extended periods

APPLICATIONS

PCR, Real-Time PCR, Southern Blotting, Mapping, RFLP

COMPONENTS

  • GENEzol™ DNA Reagent Plant
  • RNase A (50 mg/ml)

QUALITY CONTROL

GENEzol™ DNA Reagent Plant is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 50 mg of fresh Arabidopsis leaves are initially ground in GENEzol™ DNA Reagent Plant. A 15 µl aliquot of extracted genomic DNA from a 100 µl eluate is analyzed by electrophoresis on a 1% agarose gel.

GENEzol™ DNA Reagent Plant Functional Test Data

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Figure 1 .Genomic DNA (approximately 30 kb) was extracted using GENEzol™ DNA Reagent Plant. 50 mg of fresh Arabidopsis leaves were initially ground in GENEzol™ DNA Reagent Plant. A 15 µl aliquot of extracted genomic DNA from a 50 µl eluate was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 
Test
DNA Conc.
260/280
260/230
Yield
1
380.7 µg/ml
1.97
1.88
19.04 µg
2
316.9 µg/ml
1.96
1.89
15.85 µg
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Figure 2 .Genomic DNA (approximately 30 kb) was extracted using GENEzol™ DNA Reagent Plant. 50 mg of fresh rose leaves were initially ground in GENEzol™ DNA Reagent Plant. A 5 µl aliquot of extracted genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 
Test
DNA Conc.
260/280
260/230
Yield
1
109.5 µg/ml
1.82
1.71
11.0 µg
2
106.0 µg/ml
1.82
1.73
10.6 µg
3
99.4 µg/ml
1.81
1.71
9.9 µg

Reagent Genomic DNA Extraction Kits Specifications

 
SpecificationsGeneaid™ DNA Isolation Kit BloodGeneaid™ DNA Isolation Kit Cultured CellGeneaid™ DNA Isolation Kit TissueGeneaid™ DNA Isolation Kit BacteriaGeneaid™ DNA Isolation Kit YeastGENEzol™ DNA Reagent Plant
Cat. #GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GEY100
GEY300
GR100
GR200
RXNS100-1,000150-1,500150-1,500150-1,500100-300100-200
FormatReagent SystemReagent SystemReagent SystemReagent SystemReagent SystemReagent System
Sample300 µl-10 ml bloodup to 5x107 cultured cellsup to 200 mg tissueup to 1.5x109 bacterial cellsup to 2x108 yeast cellsup to 1 g plant tissue
Elution Volume100 µl-1 ml50-200 µl100-200 µl100 µl50-100 µl50-100 µl
Operation Time<40 minutes<60 minutes<70 minutes<60 minutes<40 minutes<50 minutes

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