PRODUCTS | Geneaid Biotech Ltd

Yeast DNA Isolation Kit (GEY100, GEY300)

Reagent DNA Extraction/Reagent DNA Extraction

The Geneaid™ DNA Isolation Kit (Yeast) offers a simple and gentle reagent DNA precipitation method for isolating protein free, high molecular weight genomic, mitochondrial or viral DNA from Saccharomyces cerevisiae and a variety of other yeast and fungus species. This highly versatile solution based system offers a convenient procedure with minimal hands on time. The provided Sorbitol Buffer, when combined with zymolase or lyticase, will efficiently lyse yeast and other fungus species cell walls consisting of chitin and polysaccharides. Purified DNA (A260/A280 = 1.8-2.0), is suitable for use in PCR or other enzymatic reactions.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # GEY100, GEY300)

Purify genomic DNA from (up to 2 x 10⁸) yeast and other fungus species using a simple and gentle 3 buffer system
Simple and gentle reagent DNA precipitation method
Convenient premixed Sorbitol Buffer!
Time: 40 minutes
Elution volume: 50-100 µl
Storage: dry at room temperature (15-25°C)

APPLICATIONS

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR

COMPONENTS

Sorbitol Buffer
Cell Lysis Buffer
Protein Removal Buffer
DNA Hydration Buffer

QUALITY CONTROL

The quality of the Presto™ Mini gDNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Geneaid™ DNA Isolation Kit (Yeast) Funtional Test Data

Figure 1 .Genomic DNA (approximately 30 kb) was extracted using the Geneaid™ DNA Isolation Kit (Yeast). Saccharomyces cerevisiae (2×10⁸) was harvested by centrifugation at 5,000 x g for 10 minutes. A 15 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

Test

DNA Conc.

260/280

260/230

Yield

115.5 µg/ml

1.91

1.87

11.6 µg

142.9 µg/ml

1.92

2.01

14.3 µg

137.1 µg/ml

1.91

1.97

13.7 µg

Reagent Genomic DNA Extraction Kits Specifications

Specifications	Geneaid™ DNA Isolation Kit Blood	Geneaid™ DNA Isolation Kit Cultured Cell	Geneaid™ DNA Isolation Kit Tissue	Geneaid™ DNA Isolation Kit Bacteria	Geneaid™ DNA Isolation Kit Yeast	GENEzol™ DNA Reagent Plant
Cat. #	GEB100 GEB01K GEB01K+	GEC150 GEC1.5K GEC1.5K+	GET150 GET1.5K GET1.5K+	GEE150 GEE1.5K GEE1.5K+	GEY100 GEY300	GR100 GR200
RXNS	100-1,000	150-1,500	150-1,500	150-1,500	100-300	100-200
Format	Reagent System	Reagent System	Reagent System	Reagent System	Reagent System	Reagent System
Sample	300 µl-10 ml blood	up to 5x10⁷ cultured cells	up to 200 mg tissue	up to 1.5x10⁹ bacterial cells	up to 2x10⁸ yeast cells	up to 1 g plant tissue
Elution Volume	100 µl-1 ml	50-200 µl	100-200 µl	100 µl	50-100 µl	50-100 µl
Operation Time	<40 minutes	<60 minutes	<70 minutes	<60 minutes	<40 minutes	<50 minutes

RELATED PRODUCTS

Product Name	RXNS	Catalogue Number
Geneaid™ DNA Isolation Kit (Bacteria)	150/1,500	GEE100/01K/01K+
Geneaid™ DNA Isolation Kit (Blood)	100/1,000	GEB100/01K/01K+
Geneaid™ DNA Isolation Kit (Tissue)	150/1,500	GET150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Cultured Cell)	150/1,500	GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast)	100/300	GEY100/300
GENEzol™ DNA Reagent Plant	100/200	GR100/200

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