PRODUCTS | Geneaid Biotech Ltd

Bacteria DNA Isolation Kit (GEE150, GEE1.5K)

Reagent DNA Extraction/Reagent DNA Extraction

The Geneaid™ DNA Isolation Kit (Bacteria) offers a simple and gentle reagent DNA precipitation method for isolating protein free, high molecular weight genomic and viral DNA from Gram (+) positive and Gram (-) negative bacteria cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # GEE150, GEE1.5K, GEE1.5K+)

Isolate high molecular weight DNA for archiving or sensitive downstream applications
High yield, High quality DNA (A260/A280 = 1.8-2.0)
Sample: 0.5-1.5 x 10⁹ bacteria cells
Easily scalable to satisfy larger bacteria sample volumes
Minimal hands on time
Convenient, simple and gentle reagent DNA precipitation method
Storage: dry at room temperature (15-25°C) for up to 2 years, Lysozyme is shipped at room temperature and should be stored at -20°C for up to 1 year

APPLICATIONS

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR

COMPONENTS

Cell Lysis Buffer
Gram+ Buffer
Protein Removal Buffer
DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
RNase A (10 mg/ml) - not included with GEE1.5K
Lysozyme - not included with GEE1.5K

QUALITY CONTROL

The quality of the Geneaid™ DNA Isolation Kit (Bacteria) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 10⁹ bacteria cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Reagent Genomic DNA Extraction Kits Specifications

Specifications	Geneaid™ DNA Isolation Kit Blood	Geneaid™ DNA Isolation Kit Cultured Cell	Geneaid™ DNA Isolation Kit Tissue	Geneaid™ DNA Isolation Kit Bacteria	Geneaid™ DNA Isolation Kit Yeast	GENEzol™ DNA Reagent Plant
Cat. #	GEB100 GEB01K GEB01K+	GEC150 GEC1.5K GEC1.5K+	GET150 GET1.5K GET1.5K+	GEE150 GEE1.5K GEE1.5K+	GEY100 GEY300	GR100 GR200
RXNS	100-1,000	150-1,500	150-1,500	150-1,500	100-300	100-200
Format	Reagent System	Reagent System	Reagent System	Reagent System	Reagent System	Reagent System
Sample	300 µl-10 ml blood	up to 5x10⁷ cultured cells	up to 200 mg tissue	up to 1.5x10⁹ bacterial cells	up to 2x10⁸ yeast cells	up to 1 g plant tissue
Elution Volume	100 µl-1 ml	50-200 µl	100-200 µl	100 µl	50-100 µl	50-100 µl
Operation Time	<40 minutes	<60 minutes	<70 minutes	<60 minutes	<40 minutes	<50 minutes

RELATED PRODUCTS

Product Name	RXNS	Catalogue Number
Geneaid™ DNA Isolation Kit (Bacteria)	150/1,500	GEE100/01K/01K+
Geneaid™ DNA Isolation Kit (Blood)	100/1,000	GEB100/01K/01K+
Geneaid™ DNA Isolation Kit (Tissue)	150/1,500	GET150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Cultured Cell)	150/1,500	GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast)	100/300	GEY100/300
GENEzol™ DNA Reagent Plant	100/200	GR100/200

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