旭基科技股份有限公司

Advantages (Cat. # PR050/PR100)

• Purify up to 50 µg of total RNA in 10 minutes!
• Compatibility: purify RNA stored in RNase-free water, elution buffer or TE buffer following RNA extraction using GENEzol™, TRI-Reagent®, TRIzol®, RNAzol®, QIAzol® etc.
• Sample Volume: up to 100 μl of RNA products
• Membrane: glass fiber membrane optimized for total RNA extraction
• Spin Columns: individually packaged RNA spin columns, certified RNase and DNase-free
• Elution Volume: 20-50 μl
• Recovery: up to 80% of high-quality RNA (A260/A280 = 1.9-2.0)
• Storage: room temperature (15-25ºC)

APPLICATIONS

RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protention Assay

COMPONENTS

• RNA Pure Buffer
• Wash Buffer
• RNase-free Water
• PR Columns
• 2 ml Collection Tubes

QUALITY CONTROL

The quality of the RNA Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Following RNA purifcation using the RNA Pure Kit, 10 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

RNA Pure Kit Functional Test Data

Figure 1 .Total RNA was extracted using Supplier I Tri Reagent. An Escherichia coli (1×10⁹) culture (OD600=1.3, 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. 10 µl from a 50 µl eluate of extracted RNA was analyzed by electrophoresis on a 0.8% agarose gel. Genomic DNA and other contaminants remain in the final product.

M = Geneaid 1 Kb DNA Ladder

 

Test

RNA Yield

260/280

260/230

1

21.3 µg

1.67

0.72

2

20.2 µg

1.60

0.84

3

22.4 µg

1.63

1.07