旭基科技股份有限公司

Advantages (Cat. # PL0245)

• Wide Molecular Weight range: 10-245 kDa
• Supplied buffer for direct gel loading
• Sharp bands with 28 and 75 kDa reference bands (green/red dye)
• Includes Protein Loading Dye (2 ml, 5X)
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
• 1.5~2.5 μl per well for general Western transferring
• Increase application amount for thicker or larger gel
• Optional Dithiothreitol (DTT) procedure

STORAGE

• 25ºC for 2 weeks
• 4ºC for 3 months
• -20ºC for 24 months

APPLICATIONS

Monitor protein migration/sample in SDS-PAGE, Monitor protein transfer onto membranes during Western Blotting, Sizing of proteins on SDS-PAGE and Western blots

COMPONENTS

• Prestained Protein Ladder (approximately 0.1~0.4 mg/ml of each protein in buffer {20 mM Trisphosphate pH7.5 at 25°C, 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea and 15% (v/v) glycerol}.
• Protein Loading Dye (2 ml, 5X)

QUALITY CONTROL

The quality of the Prestained Protein Ladder (245 kDa) is tested on a lot-to-lot basis according to Geneaid's ISO certified quality management system. 5 μl of Prestained Protein Ladder (245 kDa) resolves 12 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocelluslose membrane.

Prestained Protein Ladder V Guide for Molecular Weight Estimation (kDa)

Figure 1 .Migration patterns of various electrophoretic conditions (according to type and percentage of running buffer). The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoretic condition.