Maxi Plasmid DNA Kit PLUS (PM010+, PM025+)DNA Extraction/Plasmid DNA Purification
The Geneaid™ Maxi Plasmid Kit PLUS uses pre-packed anion-exchange resin columns to purify plasmid DNA from 200-1600 ml of cultured bacterial cells. This kit includes an increased volume of reagents for processing 3-6 g bacteria cell pellets. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA is bound to the column and contaminants are efficiently removed. The purified plasmid DNA is eluted then precipitated with isopropanol for desalting. The entire procedure can be completed in 100 minutes without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.