旭基科技股份有限公司

Specifications (Cat. # PME10+, PME25+)

• Includes an increased volume of reagents for processing 2.6-4 g bacteria cell pellets
• High Yield: 1.0 mg of pure transfection grade plasmid DNA from 300 ml cultured bacterial cells
• Efficient endotoxin removal (<0.1 EU/µg DNA) verified by LAL
• Large Sample Volume: 200-1100 ml of cultured bacterial cells
• Format: plasmid maxiprep anion-exchange resin column
• Elution Volume: 500 µl - 2 ml
• Operation time: within 100 minutes
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS

Transfection, Sequencing, Ligation, PCR, In-vitro Transcription Microinjection, Restriction Enzyme Digestion, Gene Gun

COMPONENTS

• PM1, PM2, PM3 Buffer
• TrueBlue Lysis Buffer
• PER Buffer
• PEQ Buffer
• PMC Buffer
• PEL Buffer
• RNase A
• Plasmid Maxi Columns

QUALITY CONTROL

The quality of the Geneaid™ Maxi Plasmid Kit PLUS (Endotoxin Free) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 300 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Maxi Plasmid Kit. Following the purification process, a yield of more than 900 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is analyzed by electrophoresis.

PPlasmid Maxi Kit Endotoxin Free Funtional Test Data

Figure 1 .Plasmid DNA was extracted using the Geneaid™ Maxi Plasmid Kit (Endotoxin Free). The purified supercoiled plasmid DNA [300 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (A600 > 2 U/ml, OD600 = 4.0)], was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 

Sample

DNA Conc.

260/280

260/230

Total Yield

Test 1

1012.2 µg/ml

1.87

2.25

1.0 mg

Test 2

1015.10 µg/ml

1.87

2.29

1.0 mg

Plasmid DNA Midi and Maxi Kits Specifications