Large Plasmid Mini Kit (PDL100, PDL300)DNA Extraction/Plasmid DNA Purification
The Large Plasmid DNA Extraction Kit was designed for plasmid and cosmid DNA purification from 1-4 ml of cultured bacterial cells. This plasmid kit is optimized for extracting 10-50 kb plasmid DNA using a miniprep spin column system. The Large Plasmid DNA Extraction Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE. Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in Restriction Enzyme Digestion, Ligation, PCR, and sequencing reactions.