Midi Spin Column Plasmid Kit (PA025)DNA Extraction/Plasmid DNA Purification
The Midiprep Spin Column Plasmid Kit was designed for plasmid and cosmid DNA purification from 50-100 ml of cultured bacterial cells using a plasmid midiprep spin column system. This plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water. Typical yields are 200-350 μg for high-copy number plasmid or 30-100 μg for low-copy number plasmid from 50 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 35 minutes.