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Presto™ Rapid Extract PCR kit (RPCR100, RPCR500)

PCR/PCR

Presto™ Rapid Extract PCR kit consists of ZEROprep™ DNA Reagent and 2X Hot Start Taq Master Mix with Dye. ZEROprep™ DNA Reagent is designed for efficient release of DNA from variety of samples for direct use in PCR reactions without purification. In addition, 2X Hot Start Taq PCR Master Mix with Dye includes all of the components necessary to perform PCR. Add primers, template DNA and sterile water to complete the PCR reaction mix in routine PCR assays. Simply place the samples in the ZEROprep™ DNA reagent, only 10 minutes incubation and transfer the sample lysate as DNA template to 2X Hot Start Taq Master Mix with Dye for PCR assay. After PCR reaction, the PCR mixture could be conveniently loaded on the agarose gel without mix with loading dye in advance.

DOCUMENTS

DESCRIPTION

Advantages (Cat. RPCR100, RPCR500)

  • Wide variety of sample types (tissue, blood, plant, bacteria, yeast/fungus, virus)
  • Use DNA directly in PCR reactions
  • Ready-to-use 2X Hot Start Taq Master Mix with DNA loading dye
  • Recombinant sourced Hot Start Taq DNA Polymerase
  • Fast PCR set up due to 2-8 ºC storage temperature which eliminates thawing time
  • Amplification of long targets: 100 bp – 5 kb
  • 3' – 5' Exonuclease Proofreading Ability: NO
  • 5' – 3' Exonoclease Activity: YES

SOURCE

The Hot Start Taq DNA Polymerase (Antibody modification) is a hot-start thermostable DNA polymerase which was expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

COMPOSITION

  • ZEROprep™ DNA Reagent
  • 2X Hot Start Taq Master Mix with Dye

UNIT DEFINITION

One unit incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 74ºC.

QUALITY CONTROL

Presto™ Rapid Extract PCR kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. DNA from a 1 mg tissue sample is lysed in ZEROprep™ DNA Reagent. A 2 μl aliquot of lysate is added directly into a 25 μl PCR mix using 2X Hot Start Taq PCR Master Mix with Dye.

Presto™ Rapid Extract PCR kit Data

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Figure 1 .Human blood samples were lysed in ZEROprep™ DNA PCR Reagent for 15 minutes at room temperature. Plant and bacteria samples were lysed for 15 minutes at 80℃. After sample lysis, 2 μl aliquots of lysate were added to 2X Hot Start Taq PCR Master Mix with Dye for PCR assays as DNA template. Lane 1-2: partial human ACTB gene (250 bp) was amplified from 10 μl of human blood. Lane 3-4: partial Actin-2 gene (750 bp) was amplified from 20 mg of Arabidopsis thaliana leaves. Lane 5: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Escherichia coli broth. Lane 6: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Bacillus subtilis broth.

Reagent Genomic DNA Extraction Kits Specifications

 
SpecificationsGeneaid™ DNA Isolation Kit BloodGeneaid™ DNA Isolation Kit Cultured CellGeneaid™ DNA Isolation Kit TissueGeneaid™ DNA Isolation Kit BacteriaGeneaid™ DNA Isolation Kit YeastGENEzol™ DNA Reagent Plant
Cat. #GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GBY100
GBY300
GR100
GR200
RXNS100-1,000150-1,500150-1,500150-1,500100-300100-200
FormatReagent SystemReagent SystemReagent SystemReagent SystemReagent SystemReagent System
Sample300 µl-10 ml bloodup to 5x107 cultured cellsup to 200 mg tissueup to 1.5x109 bacterial cellsup to 2x108 yeast cellsup to 1 g plant tissue
Elution Volume100 µl-1 ml50-200 µl100-200 µl100 µl50-100 µl50-100 µl
Operation Time<40 minutes<60 minutes<70 minutes<60 minutes<40 minutes<50 minutes

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