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Geneaid Hot Start Taq PCR Master Mix with Dye (HSTQ100)

PCR/PCR

Geneaid 2X Hot Start Taq PCR Master Mix with Dye includes all of the components necessary to perform PCR, with the exception of template and primer. Antibody modified Hot Start Taq DNA Polymerase, dNTPs, and reaction buffers are provided at optimized concentrations. Simply add primers, template DNA and sterile water to a final volume of up to 100 μl to complete the PCR reaction mix in routine PCR assays including colony PCR and recombinant screening PCR. In addition, the blue color DNA loading dye has already been mixed in the Geneaid Hot Start Taq PCR Master Mix. After PCR reaction, the PCR products could be conveniently loaded on the agarose gel without mix with loading dye in advance.

DOCUMENTS

DESCRIPTION

Advantages (Cat. HSTQ100)

  • Ready-to-use mix with DNA loading dye
  • Fast PCR set up due to 4ºC storage temperature which eliminates thawing time!
  • Recombinant sourced Hot Start Taq DNA Polymeras
  • 100 rxns (12.5 μl/rxn, 1.25 ml)
  • Concentration: 2X (100 U/ml Hot Start Taq, 0.5 mM dNTPs, 2 mM Mg2+)
  • Amplification of long targets: 100 bp – 5 kb
  • Storage: 4ºC (12 months)
  • Half life of 30 minutes at 95ºC
  • 3' – 5' Exonuclease Proofreading Ability: NO
  • 5' – 3' Exonoclease Activity: YES

SOURCE

The Hot Start Taq DNA Polymerase (Antibody modification) is a hot-start thermostable DNA polymerase which was expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

COMPOSITION

2X Buffer: 40 mM Tris-HCl, 20 mM KCl, 4 mM Mg2+, 0.2% Triton X-100, 0.5 mM dNTP, 100 Units/ml of Hot Start Taq polymerase

UNIT DEFINITION

One unit incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 74ºC.

QUALITY CONTROL

The quality of 2X Hot Start Taq Master Mix with Dye is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 2X Hot Start Taq Master Mix with Dye is used for PCR reactions to amplify a 3,000 bp region of the green fluorescent protein gene (GFP). The PCR product is checked on an ethidium bromide-stained agarose gel.

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