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PCR Cleanup Kit (DFC100/DFC300)

DNA Extraction/PCR Cleanup

The GenepHlow™ PCR Cleanup Kit was designed to recover, concentrate or purify DNA fragments from PCR or other enzymatic reactions. This PCR purification kit includes a pH indicator as an optional addition to the PB binding buffer to ensure optimal pH and facilitate DNA binding. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. The GenepHlow™ PCR Cleanup Kit is a cost effective, high performance alternative for efficient DNA fragments PCR purification.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # DFC100, DFC300)

  • High Efficiency: up to 95% recovery from PCR or other enzymatic reactions
  • Only 10 minutes PCR cleanup
  • Includes a pH Indicator for easy determination of optimal pH
  • Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high
  • Sample Size: up to 100 μl of PCR products
  • Broad Fragment Size Range: 100 bp–20 kb
  • Spin Columns: glass fiber membrane optimized for PCR cleanup
  • Elution Volume: 20-50 µl
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

COMPONENTS

  • PB Buffer
  • pH Indicator
  • 3M Sodium Acetate (pH5.0)
  • Wash Bufffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • DFH Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the GenepHlow™ PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from PCR products and analyzed by electrophoresis.

GenepHlow™ PCR Cleanup Kit Functional Test Data

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Figure 1 .PCR product DNA fragments ranging from 250 bp-10 kb were extracted using the GenepHlow™ PCR Cleanup Kit (lane 1, 2, 3). The purified DNA from a 50 µl eluate was confirmed by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1073 ng)

 
Test
DNA Conc.
260/280
Yield
Recovery
1
19.7 ng/µl
1.86
985 ng
91.8%
2
20.3 ng/µl
1.85
1015 ng
94.6%
3
20.5 ng/µl
1.86
1025 ng
95.5%

Column Types Included With the Presto™ Midi Plasmid Kit Endotoxin Free

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Figure 2.A pH indicator is included as an optional additon to the PB binding buffer to ensure optimal pH and facilitate DNA binding. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow.

PCR Cleanup Kits Specifications

 
SpecificationsGenepHlow™ PCR Cleanup KitGenepHlow™ Gel/PCR KitGenepHlow™ DNA Cleanup Maxi KitGenepHlow™ DNA Cleanup Midi KitSmall DNA Fragments Extraction KitLarge DNA Fragments Extraction KitPresto™ 96 Well PCR Cleanup Kit
Cat. #DFC100
DFC300
DFH100
DFH300
DFM010
DFM025
DFI100
DFI300
DF101
DF301
DFL100
DFL300
96DFH04
96DFH10
FormatSpin ColumnSpin ColumnMaxi Spin ColumnMidi Spin ColumnSpin ColumnSilica Matrix96 Well Plate
Binding Capacity10 µg10 µg200 µg40 µg10 µg5 µg10 µg
SamplePCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/Restriction Enzyme Digest ProductPCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/PCR ProductPCR Product
Fragment Size70 bp-20 kb70 bp-20 kb100 bp-10 kb100 bp-20 kb40-200 bp100 bp-50 kb70 bp-20 kb
pH IndicatorYesYesYesYesN/AYesN/A
RecoveryUp to 95%Up to 95%Up to 80%Up to 90%Up to 95%Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Up to 95%
Elution Volume20-50 µl20-50 µl500-1000 µl30-50 µl20-50 µl10-20 µl60-80 µl
Operation Time10 minutes<20 minutes<25 minutes10 minutes<20 minutes<20 minutes20 minutes

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