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Gel/PCR DNA Fragments Kit (DF100, DF300)

DNA Extraction/Gel Extraction

The Gel/PCR DNA Fragments Extraction Kit is a Gel Extraction Kit and PCR Cleanup Kit in 1 convenient system. This Gel Extraction Kit will recover or concentrate 70 bp-20 kb DNA fragments from agarose gel, PCR, or other enzymatic reactions. For processing 10-50 kb DNA fragments, the Large DNA Fragments Extraction Kit is available. For processing 40-200 bp DNA fragments, the Small DNA Fragments Extraction Kit is also available. The Gel/PCR DNA Fragments Extraction Kit uses chaotropic salt to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the gel/pcr spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions such as Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray and Transfection.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # DF100, DF300)

  • High Efficiency: up to 90% recovery from agarose gels and up to 95% recovery from PCR products or other enzymatic reactions
  • Convenient: gel extraction kit and PCR cleanup kit in one
  • Only 20 minute gel extraction or 10 minute PCR cleanup
  • Sample Size: up to 300 mg of agarose gel slice or up to 100 μl of PCR products
  • Broad Fragment Size Range: 70 bp–20 kb
  • Spin Columns: glass fiber membrane optimized for both gel extraction and PCR cleanup
  • Elution Volume: 20-50 µl
  • Storage: dry at room temperature (15-25ºC)

APPLICATIONS

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

COMPONENTS

  • DF Buffer
  • W1 Buffer
  • Wash Bufffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • DF Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Gel/PCR DNA Fragments Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions and analyzed by electrophoresis.

Gel/PCR DNA Extraction Kit Functional Test Data

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Figure 1 .Gel slice DNA fragments ranging from 250 bp-10 kb were extracted using the Gel/PCR DNA Fragments Extraction Kit (total 1.0 µg, 3 replicates). The purified DNA from a 50 µl eluate was analyzed by electrophoresis on a 0.8% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 
Test
DNA Conc.
260/280
Total DNA
Recovery
M
Control
----
----
1000 ng
----
1
Test 1
17.5 ng/µl
1.83
875 ng
87.5%
2
Test 2
16.4 ng/µl
1.84
820 ng
82%
3
Test 3
17.2 ng/µl
1.83
860 ng
86%
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Figure 2.PCR product DNA fragments ranging from 250 bp-10 kb were extracted using the Gel/PCR DNA Fragments Extraction Kit (total 1.1 µg, 3 replicates). The purified DNA from a 50 µl eluate was confirmed by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 
Test
DNA Conc.
260/280
Total DNA
Recovery
M
Control
----
----
1100 ng
----
1
Test 1
20.8 ng/µl
1.85
1040 ng
95%
2
Test 2
20.3 ng/µl
1.84
1015 ng
92%
3
Test 3
19.6 ng/µl
1.84
980 ng
89%
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Figure 3 .A 13 kb DNA fragment was purified from agarose gel using the Gel/PCR DNA Fragments Extraction Kit and the equivalent competitors's gel extraction kit. The purified DNA concentration from a 30 µl eluate was confirmed by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 
Test
DNA Concentration
Total DNA
Average
Recovery
1
Original 13 kb DNA Fragment (3 µg)
----
----
----
----
2
Geneaid
72.30 ng/µl
2.17 µg
3
Geneaid
70.10 ng/µl
2.10 µg
2.21 ng
74%
4
Geneaid
79.10 ng/µl
2.37 µg
 
5
Competitor
43.90 ng/µl
1.32 µg
 
6
Competitor
39.80 ng/µl
1.20 µg
1.27 ng
42%
7
Competitor
42.70 ng/µl
1.28 µg
 

Gel Extraction and PCR Cleanup Kits Specifications

 
SpecificationsGenepHlow™ Gel Extraction KitGenepHlow™ PCR Cleanup KitGenepHlow™ Gel/PCR KitGenepHlow™ DNA Cleanup Maxi KitPresto™ 96 Well Gel Extraction KitSmall DNA Fragments Extraction KitLarge DNA Fragments Extraction Kit
Cat. #DFG100
DFG300		DFC100
DFC300		DFH100
DFH300		DFM010
DFM025		96DFG04
96DFG10		DF101
DF301		DFL100
DFL300
FormatSpin ColumnSpin ColumnSpin ColumnMaxi Spin Column96 Well PlateSpin ColumnSilica Matrix
Binding Capacity10 µg10 µg10 µg200 µg10 µg10 µg5 µg
SampleAgarose Gel SlicePCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/Restriction Enzyme Digest ProductAgarose Gel SliceAgarose Gel Slice/PCR ProductAgarose Gel Slice/PCR Product
Fragment Size70 bp-20 kb70 bp-20 kb70 bp-20 kb100 bp-10 kb70 bp-20 kb40-200 bp100 bp-50 kb
pH IndicatorYesYesYesYesN/AN/AYes
RecoveryUp to 90%Up to 95%Up to 95%Up to 80%Up to 80%Up to 95%Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Elution Volume20-50 µl20-50 µl20-50 µl500-1000 µl60-80 µl20-50 µl10-20 µl
Operation Time20 minutes10 minutes<20 minutes<25 minutes45 minutes<20 minutes<20 minutes

RELATED PRODUCTS

 
Product NameRXNSCatalogue Number
GenepHlow™ Gel Extraction Kit100/300DFG100/300
GenepHlow™ PCR Cleanup Kit100/300DFC100/300
GenepHlow™ Gel/PCR Kit100/300DFH100/300
GenepHlow™ DNA Cleanup Maxi Kit10/25DFM010/25
Presto™ 96 Well PCR Cleanup Kit4 x 96/10 x 9696DFH04/10
Small DNA Fragments Extraction Kit100/300DF101/301
Large DNA Fragments Extraction Kit100/300DFL100/300
Gel/PCR DNA Fragments Extraction Kit100/300DF100/300
Presto™ 96 Well Gel Extraction Kit4 x 96/10 x 9696DFG04/10
GenepHlow™ DNA Cleanup Midi Kit100/300DFI100/300
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