旭基科技股份有限公司

Specifications (Cat. # MPD192, MPD576, MPD1920)

• Sample Volume: 1-16 ml of cultured bacterial cells
• High Yield: 6-10 μg for high-copy number plasmid from 1 ml of cultured E. coli cells
• High Purity plasmid DNA: A260/A280 =1.8-2.0, A260/230 =2.0-2.4
• Plasmid DNA Size: 1-20 kb
• Elution volume: 25-100 µl
• Manual or automated plasmid DNA isolation
• Reduce up to 80% of plastic material waste compared to spin-column based kits, more eco friendly!
• Storage: dry at room temperature (15-25°C)

APPLICATIONS

Restriction Enzyme Digestion, Library Screening, Ligation, PCR, Transformation and Sequencing Reactions

COMPONENTS

• MPD1 Buffer
• MPD2 Buffer
• MPD3 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• RNase A
• MPD Magnetic Beads

QUALITY CONTROL

The quality of the EcoExtra™  Magnetic Beads Plasmid Kit  is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript is purified using the EcoExtra™  Magnetic Beads Plasmid Kit . Following the purification process, a yield of more than 30 μg is obtained and the A260/A280 ratio is between 1.8-2.0.

EcoExtra™  Magnetic Beads Plasmid Kit Functional Test Data 

Figure 1 .Plasmid DNA was extracted using the EcoExtra™ Magnetic Beads Plasmid Kit. 5 µl aliquots of a 100 µl eluate of purified super coiled plasmid DNA from 1, 2, and 4 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (OD600 = 4 ). The purified plasmid were analyzed by electrophoresis on a 0.8% agarose gel. 

Lane M = Geneaid 1 Kb DNA Ladder,  Lane1-2 =1 ml, Lane 3-4 =2 ml, Lane 5-6 =4 ml cultured E. coli cells

 

Sample

Yield

260/280

260/230

1ml E.coli cells

6-10 µg

1.8-2.0

2.0-2.4

2ml E.coli cells

15-20 µg

1.8-2.0

2.0-2.4

4ml E.coli cells

26-32 µg

1.8-2.0

2.0-2.4

8ml E.coli cells

40-46 µg

1.8-2.0

2.0-2.4

16ml E.coli cells

70-78 µg

1.8-2.0

2.0-2.4