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Magnetic Beads gDNA Extraction Plate Kit (Blood) MBP096

Magnetic Beads DNA/RNA Extraction/Magnetic Beads gDNA kit Blood

Geneaid Magnetic Beads gDNA Extraction Plate Kit (Blood) was designed for high-throughput purification of high-quality of genomic DNA from whole blood and buffy coat samples. Genomic DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The 96 well gDNA extraction plates can be easily adapted to Geneaid SYNC Nucleic Acids Extraction System (S032) and other similar automated extractors. The purified DNA can be used in qPCR and a variety of other downstream applications.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # MBP096)

  • Purify genomic DNA from 32 blood samples in 50 minutes!
  • Sample Volume: up to 300 µl of whole blood
  • High Yield: up to 8 μg of pure genomic DNA from 200 µl of blood samples, 12 μg of pure genomic DNA from 300 µl of blood samples
  • High Purity gDNA: A260/A280 = 1.8-2.0
  • DNA Size: 20-30 kb
  • Format: 96 well extraction plates
  • Elution volume: 100 µl
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

COMPONENTS

  • Extraction plates
  • Proteinase K
  • Tip Combs

QUALITY CONTROL

The quality of Magnetic Beads gDNA Extraction Plate Kit (Blood) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA from 200 µl whole blood sample.

Magnetic Beads gDNA Extraction Plate Kit (Blood) Funtional Test Data


Figure 1 .
Genomic DNA was extracted from 200 µl human whole blood samples from 16 different donrs using the Magnetic Beads gDNA Extraction Plate Kit (Blood) with Geneaid SYNC Nucleic Acids Extraction System. 2 µl from 100 µl eluates of purified genomic DNA was analyzed by electrophoresis on a 0.8% agarose gel.
M = Geneaid 1 Kb DNA Ladder

Figure 2 .Genomic DNA was extracted from identical 200 µl human whole blood sample (16 repeats) using the Magnetic Beads gDNA Extraction Plate Kit (Blood) with Geneaid SYNC Nucleic Acids Extraction System. 1 µl of purified DNA from individual extraction was used as template for real-time qPCR to detect ACTB gene. This data showed that high reproducibility of Magnetic Beads gDNA Extraction Plate Kit (Blood). The coefficient of variation (CV) of the Ct is 0.77%.

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