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Magnetic Beads Genomic DNA Extraction Kit (Blood) MB048/MB096

Magnetic Beads DNA/RNA Extraction/Magnetic Beads gDNA kit Blood

The Magnetic Beads Genomic DNA Extraction Kit Blood was designed specifically for efficient genomic DNA purification from blood and buffy coat. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Genomic DNA Extraction Kit Blood can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and Southern Blotting.

DOCUMENTS

DESCRIPTION

Advantages (Cat. # MB048, MB096)

  • High Yield: up to 10 µg of Genomic DNA
  • High Quality DNA: A260/A280 = 1.8-2.0
  • Magnetic Bead Concentration: 50 mg/ml
  • Magnetic Bead Size: ~ 5 µm
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Sample: 100 to 400 µl of whole blood and buffy coat (5 x 106 WBC)
  • Manual or automated DNA isolation
  • Operation time: within 35 minutes (manual) 
  • Storage: dry at room temperature (15-25ºC), Protease should be stored dry at 2-8ºC for extended periods

APPLICATIONS

qPCR, PCR, Real-time PCR, Automated Fluorescent DNA Sequencing, Southern Blotting, Next Generation Sequencing (NGS)

COMPONENTS

  • MB1 Buffer
  • MB2 Buffer
  • Protease
  • MW1 Buffer
  • MW2 Buffer
  • MB Magnetic Beads
  • Elution Buffer

QUALITY CONTROL

The quality of the The Magnetic Beads Genomic DNA Extraction Kit Blood is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA from a 300 µl whole blood sample.

Magnetic Beads Genomic DNA Extraction Kit (Blood) Functional Test Data

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Figure 1.5 µl aliquots of extracted DNA from a 100 µl eluate were analyzed on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder, Lane 1-3 = 100 µl blood sample, Lane 4-6 = 200 µl blood sample, Lane 7-9 = 300 µl blood sample, Lane 10-12 = 400 µl blood sample.

 
Sample
Volume
# of WBC
Yield
260/280
260/230
Whole Blood
100 µl
5.0 x 105
2.5-3.0 µg
> 1.8
> 1.2
200 µl
1.0 x 106
5.5-6.0 µg
> 1.8
> 1.6
300 µl
1.5 x 106
7.5-8.0 µg
> 1.8
> 1.7
400 µl
2.0 x 106
9.5-10.0 µg
> 1.8
> 1.8

Table 1 .Yield of purified DNA from 100-400 µl whole blood samples.

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Figure 2.Extracted DNA products were used as a DNA template for amplifying partial human ACTB gene. 3 μl aliquots of PCR product were loaded in each well. A 250 bp ACTB gene fragment was successfully amplified from each DNA product. M1-2: Geneaid 1 Kb DNA Ladder, Lane 1-3: DNA from 100 µl blood sample, Lane 4-6: DNA from 200 µl blood sample, Lane 7-9: DNA from 300 µl blood sample, Lane 10-12: DNA from 400 µl blood sample

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