旭基科技股份有限公司

Advantages (Cat. # MC048, MC096)

• High Yield: >70% recovery
• High Quality DNA: A260/A280 = 1.8-2.0
• Magnetic Bead Concentration: 50 mg/ml
• Magnetic Bead Size: ~ 5 µm
• Broad Fragment Size Range: 100 bp–10 kb
• Easily adapted to automated magnetic bead separation instruments and workstations
• Sample: up to 100 µl of PCR products
• Manual or automated DNA isolation
• Operation time: within 20 minutes (manual) 
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

COMPONENTS

• MC Buffer
• MW1 Buffer
• MW2 Buffer
• MC Magnetic Beads
• Elution Buffer

QUALITY CONTROL

The quality of the The Magnetic Beads PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from PCR products and analyzed by electrophoresis.

Magnetic Beads PCR Cleanup Kit Functional Test Data

Figure 1 .PCR product DNA fragments ranging from 250 bp-10 kb were extracted using the Magnetic Beads PCR Cleanup Kit (lane 1, 2, 3). The purified DNA from a 50 µl eluate was confirmed by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder (control, total DNA = 3 µg)

 

Test

DNA Conc.

260/280

Yield

Recovery

1

43.0 ng/µl

1.81

2.15

71.7%

2

42.2 ng/µl

1.81

2.11

70.3%

3

42.6 ng/µl

1.82

2.13

71.0%

Table 1 .Recovery of purified DNA fragments from 100 µl of PCR product.