PRODUCTS | Geneaid Biotech Ltd

Urine DNA Extraction Kit (UR050, UR100)

DNA Extraction/Genomic DNA Purification

The Presto™ Urine DNA Extraction Kit is designed for rapid isolation of high-quality cellular and cell-free DNA from up to 5 ml of urine. The kit includes uniquely designed Column Extension Tubes which allow for increased sample volume and yield. Urine samples are lysed using Proteinase K and a buffer containing chaotropic salt. The lysate is mixed with a binding buffer to facilitate DNA binding to the column membrane. The column is then washed and DNA is eluted with Elution Buffer. The entire procedure can be completed within 60 minutes and the purified DNA is ready for use in a variety of downstream applications such as qPCR, Next-Generation sequencing and DNA methylation analysis.

DOCUMENTS

COMPLETE PROTOCOL

DESCRIPTION

SPECIFICATIONS

Operation time: Purify cellular and cell-free DNA within 60 minutes.
Sample: 1-5 ml of urine
Format: Spin columns combined with column extension tubes using vacuum or centrifuge
Yield: 1-20 ng of DNA per ml of male urine. 5-1000 ng of DNA per ml of female urine.
Elution Volume: 30-50 µl
Storage: dry at room temperature (20~25ºC)

APPLICATIONS

qPCR, Next-Generation sequencing and DNA methylation analysis

CAUTION

During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

COMPONENTS

UR1 Buffer
UR2 Buffer
Proteinase K
W1 Buffer
Wash Buffer
Elution Buffer
Carrier RNA
Column Extender
GD Column
2 ml Collection Tube

QUALITY CONTROL

The quality of the Presto™ Urine Extraction Kit is tested on a lot-to-lot basis by isolating DNA from 2 ml of urine. Following the purification process, the purified DNA integrity was assessed by qPCR.

Presto™ Urine DNA Extraction Kit Functional Test Data

Figure 1 .Cellular and cell-free DNA was effectively purified from 5 ml of healthy female urine using Presto™ Urine DNA Extraction Kit.
The purified DNA was eluted with 30 µl Elution Buffer. Purified DNA was analyzed using the Qsep100 capillary electrophoresis system (Bioptic).

Figure 2 .Total genomic DNA was isolated from 2, 5 ml of female urine samples (2 replicates of each sample) using Presto™ Urine DNA Extraction Kit.

The purified DNA was eluted with 30 μl Elution Buffer. A real-time PCR assay was then performed with 1 μl of purified DNA as template, primers (designed to amplify the human GAPDH gene), and Fast SYBR Green PCR Master Mix using the StepOnePlus™ Real-Time PCR system (Applied Biosystems). The red line corresponds to the purified DNA from 5 ml of urine samples, the green line corresponds to the purified DNA from 2 ml of urine samples. The black line corresponds to the No Template Control.

Genomic DNA Extraction Kits Specifications

Specifications	gSYNC™ DNA Extraction Kit	Genomic DNA Mini Kit Blood Cultured Cell	Genomic DNA Mini Kit Tissue	Genomic DNA Mini/Maxi Kit Plant	Presto™ Mini gDNA Bacteria Kit	Presto™ Mini gDNA Yeast Kit	Geneius™ Micro gDNA Extraction Kit	Presto™ Buccal Swab gDNA Extraction Kit
Cat. #	GS100 GS300	GB100 GB300	GT050 GT100 GT300	GP100 GPM010 GPM020	GBB100/101 GBB300/301	GBYB100 GBYB300	GMB100 GMB300	GSK100 GSK300
Format	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column
Binding Capacity	50 µg	50 µg	50 µg	50 µg (Mini) 500 µg (Maxi)	50 µg	N/A	50 µg	50 µg
Sample	200 µl blood	300 µl blood	30 mg animal tissue	Mini: 100 mg plant tissue	1x10⁹ bacterial cells	2x10⁸ yeast cells	1-100 µl blood	buccal swabs
	20 mg animal tissue	200 µl buffy coat		Maxi: 1 g plant tissue	200 µl blood		FTA® Cards
	1x10⁷ cultured cells	1x10⁷ cultured cells			1 ml urine, plasma		1 ml urine
	hair	2x10⁸ yeast/fungus					1-100 µl saliva
Micropestle	N/A	N/A	Yes	N/A	N/A	N/A	N/A	N/A
Filter Column	N/A	N/A	N/A	Yes	N/A	N/A	N/A	Yes
Enzyme	Proteinase K	N/A	Proteinase K	N/A	Lysozyme Proteinase K	N/A	Proteinase K	Proteinase K
Typical Yield	5 µg from 200 µl blood	4-10 µg from 300 µl blood	10-40 µg from 20 mg tissue	3-5 µg from 100 mg Arabidopsis leaf (Mini)	37 µg from 1x10⁹ E. coli	10 µg from 2x10⁸ S. cerevisiae cells	300 ng from 6 mm blood spots	2 µg per swab
	10-40 µg from 20 mg tissue			80 µg from 500 mg Lemon leaf (Maxi)	10-15 µg from 1x10⁹ Bacillus subtilis
Elution Volume	30-100 µl	30-200 µl	30-200 µl	30-200 µl (Mini) 1 ml (Maxi)	30-200 µl	30-200 µl	30-100 µl	50-100 µl
Operation Time	<20 minutes	<25 minutes	30 minutes	<30 minutes	<30 minutes	<40 minutes	<20 minutes	<20 minutes

RELATED PRODUCTS

Product Name	RXNS	Catalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)	100, 300	GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)	100, 300	GB100/300
Genomic DNA Maxi Kit (Plant)	10, 25	GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)	10, 25	GDM10/25
Genomic DNA Mini Kit (Tissue)	50, 100, 300	GT050/100/300
Genomic DNA Mini Kit (Plant)	100	GP100
Geneius™ Micro gDNA Extraction Kit	100	GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit	100	GSK100/300
Presto™ Mini gDNA Yeast Kit	100, 300	GBYB100/300
Presto™ Mini gDNA Bacteria Kit	100, 300	GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GPP04/10

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