Stool DNA Extraction Kit (STLD050, STLD100)DNA Extraction/Genomic DNA Purification
The Presto™ Stool gDNA Extraction Kit is designed for rapid isolation of genomic DNA from microorganisms, such as bacteria and fungi in stool samples. The stool sample is homogenized and disrupted using a lysis buffer and ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a proprietary inhibitor removal buffer. Residual inhibitors are further removed by passing through a specialized PCR inhibitor removal column. Genomic DNA in the sample is then bound by the GD Column followed by wash and elution. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.