PRODUCTS | Geneaid Biotech Ltd

Stool DNA Extraction Kit (STLD050, STLD100)

DNA Extraction/Genomic DNA Purification

The Presto™ Stool gDNA Extraction Kit is designed for rapid isolation of genomic DNA from microorganisms, such as bacteria and fungi in stool samples. The stool sample is homogenized and disrupted using a lysis buffer and ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a proprietary inhibitor removal buffer. Residual inhibitors are further removed by passing through a specialized PCR inhibitor removal column. Genomic DNA in the sample is then bound by the GD Column followed by wash and elution. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # STLD050, STLD100)

Purify genomic DNA from stool samples within 50 minutes!
Sample: 180-200 mg of fresh or frozen stool samples
Binding Capacity: 50 µg
High Quality DNA: A260/A280 = 1.8-2.0
Format: beadbeating tubes, inhibitor removal columns and spin columns
Elution Volume: 30-100 µl
Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

ST1 Buffer
ST2 Buffer
ST3 Buffer
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
Inhibitor Removal Columns
GD Columns
Beadbeating Tubes Type C
2 ml Centrifuge Tubes
2 ml Collection Tubes

QUALITY CONTROL

The quality of the Presto™ Stool DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 mg stool samples. Following the purification process, the purified genomic DNA (A260/A280 ratio between 1.8-2.0) is analyzed by electrophoresis.

Presto™ Stool DNA Extraction Kit Functional Test Data

Figure 1 .Total DNA was purified from 150 mg of human stool samples which were frozen at -20ºC for 1 week and from 80 mg of rabbit stool samples which were stored at room temperature for 1 week using the Presto™ Stool DNA Extraction Kit. 5 µl aliquots of purified DNA from 100 µl eluates were analyzed on a 0.8% agarose gel.

M = Geneaid 1 Kb DNA Ladder
Lanes 1-2 = Human stool samples
Lanes 3-4 = Rabbit stool samples

Sample

ng/µl

Yield

260/280

260/230

127.1

12.7 µg

1.86

2.12

129.9

13.0 µg

1.87

2.22

77.6

7.7 µg

1.85

2.15

82.6

8.3 µg

1.85

2.15

Figure 3 .5 μl of undiluted, purified DNA from human and rabbit stool, served as a template for amplification of a 1.5 kb fragment from the bacterial 16S rRNA gene. The extracted DNA works undiluted in a PCR reaction, indicating the successful removal of PCR inhibitors.

M = Geneaid 1 Kb DNA Ladder
Lanes 1-2 = Human stool samples
Lanes 3-4 = Rabbit stool samples

Genomic DNA Extraction Kits Specifications

Specifications	gSYNC™ DNA Extraction Kit	Genomic DNA Mini Kit Blood Cultured Cell	Genomic DNA Mini Kit Tissue	Genomic DNA Mini/Maxi Kit Plant	Presto™ Mini gDNA Bacteria Kit	Presto™ Mini gDNA Yeast Kit	Geneius™ Micro gDNA Extraction Kit	Presto™ Buccal Swab gDNA Extraction Kit
Cat. #	GS100 GS300	GB100 GB300	GT050 GT100 GT300	GP100 GPM010 GPM020	GBB100/101 GBB300/301	GBYB100 GBYB300	GMB100 GMB300	GSK100 GSK300
Format	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column
Binding Capacity	50 µg	50 µg	50 µg	50 µg (Mini) 500 µg (Maxi)	50 µg	N/A	50 µg	50 µg
Sample	200 µl blood	300 µl blood	30 mg animal tissue	Mini: 100 mg plant tissue	1x10⁹ bacterial cells	2x10⁸ yeast cells	1-100 µl blood	buccal swabs
	20 mg animal tissue	200 µl buffy coat		Maxi: 1 g plant tissue	200 µl blood		FTA® Cards
	1x10⁷ cultured cells	1x10⁷ cultured cells			1 ml urine, plasma		1 ml urine
	hair	2x10⁸ yeast/fungus					1-100 µl saliva
Micropestle	N/A	N/A	Yes	N/A	N/A	N/A	N/A	N/A
Filter Column	N/A	N/A	N/A	Yes	N/A	N/A	N/A	Yes
Enzyme	Proteinase K	N/A	Proteinase K	N/A	Lysozyme Proteinase K	N/A	Proteinase K	Proteinase K
Typical Yield	5 µg from 200 µl blood	4-10 µg from 300 µl blood	10-40 µg from 20 mg tissue	3-5 µg from 100 mg Arabidopsis leaf (Mini)	37 µg from 1x10⁹ E. coli	10 µg from 2x10⁸ S. cerevisiae cells	300 ng from 6 mm blood spots	2 µg per swab
	10-40 µg from 20 mg tissue			80 µg from 500 mg Lemon leaf (Maxi)	10-15 µg from 1x10⁹ Bacillus subtilis
Elution Volume	30-100 µl	30-200 µl	30-200 µl	30-200 µl (Mini) 1 ml (Maxi)	30-200 µl	30-200 µl	30-100 µl	50-100 µl
Operation Time	<20 minutes	<25 minutes	30 minutes	<30 minutes	<30 minutes	<40 minutes	<20 minutes	<20 minutes

RELATED PRODUCTS

Product Name	RXNS	Catalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)	100, 300	GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)	100, 300	GB100/300
Genomic DNA Maxi Kit (Plant)	10, 25	GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)	10, 25	GDM10/25
Genomic DNA Mini Kit (Tissue)	50, 100, 300	GT050/100/300
Genomic DNA Mini Kit (Plant)	100	GP100
Geneius™ Micro gDNA Extraction Kit	100	GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit	100	GSK100/300
Presto™ Mini gDNA Yeast Kit	100, 300	GBYB100/300
Presto™ Mini gDNA Bacteria Kit	100, 300	GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GPP04/10

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