旭基科技股份有限公司

Advantages (Cat. RPCR100, RPCR500)

• Wide variety of sample types (tissue, blood, plant, bacteria, yeast/fungus, virus)
• Use DNA directly in PCR reactions
• Ready-to-use 2X Hot Start Taq Master Mix with DNA loading dye
• Recombinant sourced Hot Start Taq DNA Polymerase
• Fast PCR set up due to 2-8 ºC storage temperature which eliminates thawing time
• Amplification of long targets: 100 bp – 5 kb
• 3' – 5' Exonuclease Proofreading Ability: NO
• 5' – 3' Exonoclease Activity: YES

SOURCE

The Hot Start Taq DNA Polymerase (Antibody modification) is a hot-start thermostable DNA polymerase which was expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.

COMPOSITION

• ZEROprep™ DNA Reagent
• 2X Hot Start Taq Master Mix with Dye

UNIT DEFINITION

One unit incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 74ºC.

QUALITY CONTROL

Presto™ Rapid Extract PCR kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. DNA from a 1 mg tissue sample is lysed in ZEROprep™ DNA Reagent. A 2 μl aliquot of lysate is added directly into a 25 μl PCR mix using 2X Hot Start Taq PCR Master Mix with Dye.

Presto™ Rapid Extract PCR kit Data

Figure 1 .Human blood samples were lysed in ZEROprep™ DNA PCR Reagent for 15 minutes at room temperature. Plant and bacteria samples were lysed for 15 minutes at 80℃. After sample lysis, 2 μl aliquots of lysate were added to 2X Hot Start Taq PCR Master Mix with Dye for PCR assays as DNA template. Lane 1-2: partial human ACTB gene (250 bp) was amplified from 10 μl of human blood. Lane 3-4: partial Actin-2 gene (750 bp) was amplified from 20 mg of Arabidopsis thaliana leaves. Lane 5: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Escherichia coli broth. Lane 6: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Bacillus subtilis broth.

Reagent Genomic DNA Extraction Kits Specifications