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Tissue Genomic DNA Mini Kit (GT050, GT100, GT300)

DNA Extraction/Genomic DNA Purification

The Genomic DNA Mini Kit (Tissue) is a genomic DNA Extraction Kit optimized for genomic, mitochondrial and virus DNA purification from a variety of animal tissue and insects. This genomic DNA extraction kit includes Micropestles to ensure efficient homogenization of tissue samples and to shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fiber matrix of the genomic DNA spin column. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer, TE or water and is ready for use in a variety of downstream applications such as PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR. For RNA extraction from tissue samples, the Total RNA Mini Kit (Tissue) is also available.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # GT050, GT100, GT300)

  • Purify genomic DNA from tissue samples in 30 minutes!
    High Yield: 10-20 µg (0.5 cm mouse tail), 20-50 µg (20 mg mouse kidney)
  • Sample Size: up to 30 mg of tissue (tailsnips, liver, kidney, brain, earpunches, insects etc.)
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-100 µl
  • Provided Micropestles are certified DNase and RNase-free
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

  • Micropestle
  • GT Buffer
  • GBT Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Proteinase K
  • GS Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Genomic DNA Mini Kit (Tissue) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from a 20 mg mouse liver sample is extracted using the Genomic DNA Mini Kit (Tissue). The purified DNA (10 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Genomic DNA Mini Kit (Tissue) Functional Test Data

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Figure 1 .Genomic DNA from a variety of tissue samples was extracted using the Genomic DNA Mini Kit (Tissue). The purified genomic DNA (20-30 kb) was EcoRI digested and analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 
Mouse Tissue
Tissue Size
Total Yield
Tail
0.5 cm
10-20 µg
Liver
20 mg
10-20 µg
Brain
20 mg
10-20 µg
Lung
20 mg
5-10 µg
Muscle
20 mg
5-10 µg
Kidney
20 mg
20-50 µg

Genomic DNA Extraction Kit Specifications

 
SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300		GB100
GB300		GT050
GT100
GT300		GP100
GPM010
GPM020		GBB100/101
GBB300/301		GBYB100
GBYB300		GMB100
GMB300		GSK100
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg50 µg (Mini)
500 µg (Maxi)		50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
 20 mg animal tissue200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
 1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
 hair2x108 yeast/fungus    1-100 µl saliva 
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme Proteinase KN/AProteinase KProteinase K
Typical Yield5 µg from
200 µl blood		4-10 µg from
300 µl blood		10-40 µg from
20 mg tissue		3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
 10-40 µg from
20 mg tissue		  80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109 Bacillus subtilis   
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)		30-200 µl30-200 µl30-100 µl50-100 µl
Operation Time<20 minutes<25 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes

RELATED PRODUCTS

 
Product NameRXNSCatalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)100, 300GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)100, 300GB100/300
Genomic DNA Maxi Kit (Plant)10, 25GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)10, 25GDM10/25
Genomic DNA Mini Kit (Tissue)50, 100, 300GT050/100/300
Genomic DNA Mini Kit (Plant)100GP100
Geneius™ Micro gDNA Extraction Kit100GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit100GSK100/300
Presto™ Mini gDNA Yeast Kit100, 300GBYB100/300
Presto™ Mini gDNA Bacteria Kit100, 300GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit4 x 96, 10 x 9696GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit4 x 96, 10 x 9696GPP04/10
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