Presto™ Sperm DNA Extraction Kit (GSP100/GSP300)DNA Extraction/Genomic DNA Purification
This miniprep DNA extraction kit was designed for sperm DNA purification from up to 100 µl of semen. Proteinase K and a unique sperm lysis buffer are used to lyse sperm cells and degrade protein, allowing DNA to efficiently bind to the glass fiber matrix of the spin column. Contaminants are removed using a wash buffer and the purified DNA is eluted by a low salt elution buffer, TE or water. Following cell lysis, the procedure can be completed in as little as 10 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA is suitable for use in PCR or a variety of other enzymatic reactions.