PRODUCTS | Geneaid Biotech Ltd

Plant Genomic DNA Maxi Kit (GPM010, GPM025)

DNA Extraction/Genomic DNA Purification

The Genomic DNA Maxi Kit (Plant) is a genomic DNA extraction maxiprep kit optimized for genomic DNA, mitochondrial DNA and chloroplast DNA purification from plant tissue. This DNA extraction kit processes plant samples by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the maxiprep spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. DNA phenol extraction or alcohol precipitation is not required, and the entire procedure can be completed in less than 50 minutes.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # GPM010, GPM025)

Purify genomic DNA in less than 50 minutes!
Binding Capacity: 500 µg
Sample Size: up to 1 g (fresh plant tissue), up to 250 mg (dry plant tissue)
Maxiprep Spin Columns: glass fiber membrane optimized for genomic DNA extraction
Elution Volume: 200 µl ~ 2 ml
Storage: dry at room temperature (15-25ºC)

APPLICATIONS

PCR, Real-time PCR, Southern Blotting and RFLP

COMPONENTS

GPX1 Buffer (optimized for samples with high polysaccharide content)
GP1, GP2, GP3 Buffers
RNase A
W1 Buffer
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
Filter Column
GD Maxi Column
Collection Tube

QUALITY CONTROL

The quality of the Genomic DNA Maxi Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is extracted from 500 mg fresh young leaf samples. The purified genomic DNA (>80 µg) is quantified with a spectrophotometer and analyzed by electrophoresis.

Genomic DNA Maxi Kit (Plant) Functional Test Data

Figure 1 .Genomic DNA (approximately 30 kb) was extracted using the Genomic DNA Maxi Kit (Plant). 0.5 g of fresh lemon leaves and 0.5 g of fresh coffee leaves were ground to a fine powder followed by DNA extraction. 2 µl aliquots from a 1 ml eluate were analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA ladder

Sample

DNA Conc.

260/280

Yield

1. Lemon Leaf

80.6 ng/µl

1.81

80.6 µg

2. Lemon Leaf

82.2 ng/µl

1.80

82.2 µg

3. Coffee Leaf

37.3 ng/µl

1.79

37.3 µg

4. Coffee Leaf

36.4 ng/µl

1.75

36.4 µg

Genomic DNA Extraction Kits Specifications

Specifications	gSYNC™ DNA Extraction Kit	Genomic DNA Mini Kit Blood Cultured Cell	Genomic DNA Mini Kit Tissue	Genomic DNA Mini/Maxi Kit Plant	Presto™ Mini gDNA Bacteria Kit	Presto™ Mini gDNA Yeast Kit	Geneius™ Micro gDNA Extraction Kit	Presto™ Buccal Swab gDNA Extraction Kit
Cat. #	GS100 GS300	GB100 GB300	GT050 GT100 GT300	GP100 GPM010 GPM020	GBB100/101 GBB300/301	GBYB100 GBYB300	GMB100 GMB300	GSK100 GSK300
Format	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Reagent System	Spin Column	Spin Column
Binding Capacity	50 µg	50 µg	50 µg	50 µg (Mini) 500 µg (Maxi)	50 µg	N/A	50 µg	50 µg
Sample	200 µl blood	300 µl blood	30 mg animal tissue	Mini: 100 mg plant tissue	1x10⁹ bacterial cells	2x10⁸ yeast cells	1-100 µl blood	buccal swabs
	20 mg animal tissue	200 µl buffy coat		Maxi: 1 g plant tissue	200 µl blood		FTA® Cards
	1x10⁷ cultured cells	1x10⁷ cultured cells			1 ml urine, plasma		1 ml urine
	hair	2x10⁸ yeast/fungus					1-100 µl saliva
Micropestle	N/A	N/A	Yes	N/A	N/A	N/A	N/A	N/A
Filter Column	N/A	N/A	N/A	Yes	N/A	N/A	N/A	Yes
Enzyme	Proteinase K	N/A	Proteinase K	N/A	Lysozyme Proteinase K	Lyticase or Zymolase	Proteinase K	Proteinase K
Typical Yield	5 µg from 200 µl blood	4-10 µg from 300 µl blood	10-40 µg from 20 mg tissue	3-5 µg from 100 mg Arabidopsis leaf (Mini)	37 µg from 1x10⁹ E. coli	10-15 µg from 2x10⁸ S. cerevisiae cells	300 ng from 6 mm blood spots	2 µg per swab
	10-40 µg from 20 mg tissue			80 µg from 500 mg Lemon leaf (Maxi)	10-15 µg from 1x10⁹ Bacillus subtilis
Elution Volume	30-100 µl	30-200 µl	30-200 µl	30-200 µl (Mini) 1 ml (Maxi)	30-200 µl	50-100 µl	30-100 µl	50-100 µl
Operation Time	<20 minutes	<25 minutes	30 minutes	<30 minutes	<30 minutes	<40 minutes	<20 minutes	<20 minutes

RELATED PRODUCTS

Product Name	RXNS	Catalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)	100, 300	GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)	100, 300	GB100/300
Genomic DNA Maxi Kit (Plant)	10, 25	GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)	10, 25	GDM10/25
Genomic DNA Mini Kit (Tissue)	50, 100, 300	GT050/100/300
Genomic DNA Mini Kit (Plant)	100	GP100
Geneius™ Micro gDNA Extraction Kit	100	GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit	100	GSK100/300
Presto™ Mini gDNA Yeast Kit	100, 300	GBYB100/300
Presto™ Mini gDNA Bacteria Kit	100, 300	GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GPP04/10

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