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Plant Genomic DNA Maxi Kit (GPM010, GPM025)

DNA Extraction/Genomic DNA Purification

The Genomic DNA Maxi Kit (Plant) is a genomic DNA extraction maxiprep kit optimized for genomic DNA, mitochondrial DNA and chloroplast DNA purification from plant tissue. This DNA extraction kit processes plant samples by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the maxiprep spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. DNA phenol extraction or alcohol precipitation is not required, and the entire procedure can be completed in less than 50 minutes.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # GPM010, GPM025)

  • Purify genomic DNA in less than 50 minutes!
  • Binding Capacity: 500 µg
  • Sample Size: up to 1 g (fresh plant tissue), up to 250 mg (dry plant tissue)
  • Maxiprep Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 200 µl ~ 2 ml
  • Storage: dry at room temperature (15-25ºC)

APPLICATIONS

PCR, Real-time PCR, Southern Blotting and RFLP

COMPONENTS

  • GPX1 Buffer (optimized for samples with high polysaccharide content)
  • GP1, GP2, GP3 Buffers
  • RNase A
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Filter Column
  • GD Maxi Column
  • Collection Tube

QUALITY CONTROL

The quality of the Genomic DNA Maxi Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is extracted from 500 mg fresh young leaf samples. The purified genomic DNA (>80 µg) is quantified with a spectrophotometer and analyzed by electrophoresis.

Genomic DNA Maxi Kit (Plant) Functional Test Data

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Figure 1 .Genomic DNA (approximately 30 kb) was extracted using the Genomic DNA Maxi Kit (Plant). 0.5 g of fresh lemon leaves and 0.5 g of fresh coffee leaves were ground to a fine powder followed by DNA extraction. 2 µl aliquots from a 1 ml eluate were analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA ladder

 
Sample
DNA Conc.
260/280
Yield
1. Lemon Leaf
80.6 ng/µl
1.81
80.6 µg
2. Lemon Leaf
82.2 ng/µl
1.80
82.2 µg
3. Coffee Leaf
37.3 ng/µl
1.79
37.3 µg
4. Coffee Leaf
36.4 ng/µl
1.75
36.4 µg

Genomic DNA Extraction Kits Specifications

 
SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM020
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnReagent SystemSpin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg50 µg (Mini)
500 µg (Maxi)
50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
 20 mg animal tissue200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
 1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
 hair2x108 yeast/fungus    1-100 µl saliva 
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme Proteinase KLyticase or ZymolaseProteinase KProteinase K
Typical Yield5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10-15 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
 10-40 µg from
20 mg tissue
  80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109 Bacillus subtilis   
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)
30-200 µl50-100 µl30-100 µl50-100 µl
Operation Time<20 minutes<25 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes

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