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Plant Genomic DNA Mini Kit (GP100)

DNA Extraction/Genomic DNA Purification

The Genomic DNA Mini Kit (Plant) is a genomic DNA extraction kit optimized for genomic DNA, mitochondrial DNA and chloroplast DNA purification from plant tissue. This DNA extraction kit processes plant samples by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. DNA phenol extraction or alcohol precipitation is not required, and the entire procedure can be completed in less than 30 minutes. For RNA purification from plant samples, the Total RNA Mini Kit (Plant) is also available.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # GP100)

  • Purify genomic DNA in less than 30 minutes!
  • High Yield: 3-5 μg (100 mg Thale cress), 20-25 µg (100 mg Tobacco)
  • Sample Size: up to 100 mg (fresh plant tissue), up to 25 mg (dry plant tissue)
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-200 µl
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, Real-time PCR, Southern Blotting and RFLP

COMPONENTS

  • GPX1 Buffer (optimized for samples with high polysaccharide content)
  • GP1, GP2, GP3 Buffers
  • RNase A
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Filter Column
  • GD Column
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Genomic DNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is extracted from 50 mg fresh young leaf samples. The purified genomic DNA (>10 µg) is quantified with a spectrophotometer and checked by electrophoresis.

Genomic DNA Mini Kit (Plant) Interactive Protocol

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Figure 1 .Genomic DNA was extracted from a variety of 100 mg plant sample species using the Genomic DNA Mini Kit (Plant). A 3 µl aliquot from each 200 µl eluate was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder


1. Cinnamomun camphora (Camphor tree)
2. Pisum sativum (Pea sprout)
3. Arabidopsis thaliana (Thale cress)
4. Oryza sativa (Rice)
5. Ipomoea batatas (Sweet potato vine)
6. Rhizoma dioscoreae (Chinese yam )
7. Populus tremula (Aspen)
8. Flammulina velutipes (Mushroom)
9. Oxalis comiculats (Clover)

 
Plant Species
DNA Yield
Thale Cress (Arabidopsis thaliana)
3~5 µg
Rice (Oryza sativa)
10~15 µg
Tomato (Lycopersicon esculentum)
10~15 µg
Chinese Yam (Rhizoma dioscoreae)
30~60 µg
Com (Zea mays)
15~20 µg
Sweet Potato (lpomoea batatas)
20~30 µg
Orchid (Phalaenopsis aphrodite)
5~10 µg
Camphor (Cinnamommun camphora)
15~20 µg
Spinach (Spinacia oleracea)
5~10 µg
Bamboo (Bambusa iodhamii)
10~15 µg

Table 1 .The approximate genomic DNA yield from a variety of plant species using the Genomic DNA Mini Kit (Plant).

Genomic DNA Extraction Kits Specifications

 
SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300		GB100
GB300		GT050
GT100
GT300		GP100
GPM010
GPM020		GBB100/101
GBB300/301		GBYB100
GBYB300		GMB100
GMB300		GSK10
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg50 µg (Mini)
500 µg (Maxi)		50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
 20 mg animal tissue200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
 1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
 hair2x108 yeast/fungus    1-100 µl saliva 
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme Proteinase KN/AProteinase KProteinase K
Typical Yield5 µg from
200 µl blood		4-10 µg from
300 µl blood		10-40 µg from
20 mg tissue		3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
 10-40 µg from
20 mg tissue		  80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109 Bacillus subtilis   
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)		30-200 µl30-200 µl30-100 µl50-100 µl
Operation Time<20 minutes<20 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes

RELATED PRODUCTS

 
Product NameRXNSCatalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)100, 300GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)100, 300GB100/300
Genomic DNA Maxi Kit (Plant)10, 25GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)10, 25GDM10/25
Genomic DNA Mini Kit (Tissue)50, 100, 300GT050/100/300
Genomic DNA Mini Kit (Plant)100GP100
Geneius™ Micro gDNA Extraction Kit100GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit100GSK100/300
Presto™ Mini gDNA Yeast Kit100, 300GBYB100/300
Presto™ Mini gDNA Bacteria Kit100, 300GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit4 x 96, 10 x 9696GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit4 x 96, 10 x 9696GPP04/10
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