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Dry Blood Spot DNA Kit (GMB100, GMB300)

DNA Extraction/Genomic DNA Purification

The Geneius™ Micro gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic and mitochondrial DNA) from small volumes of whole blood, dried blood spots (Whatman® FTA® Cards), urine and saliva. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Carrier RNA is included with this DNA extraction kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in as little as 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 150-200 ng from 6 mm dried blood spots. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # GMB100, GMB300)

  • Purify genomic DNA from small volume blood samples (1 µl), dried blood spots (Whatman® FTA® Cards), urine, saliva
  • Time: in as little as 20 minutes
  • High Yield: Up to 300 ng of pure genomic DNA per 6 mm diameter punch from Whatman® FTA® Cards (dried blood spots)
  • High Purity gDNA: A260/A280 = 1.8-2.0
  • DNA Size: 20-30 kb
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

COMPONENTS

  • S1 Buffer
  • S2 Buffer
  • Carrier RNA
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Proteinase K
  • GD Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Geneius™ Micro gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (150-200 ng with an A260/A280 ratio of 1.8-2.0) from 10 µl blood samples is quantified with a spectrophotometer and analyzed by electrophoresis.

Real-Time PCR Data

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Figure 1 .Genomic DNA was extracted from blood samples (1–64 µl) using the Geneius™ Micro gDNA Extraction Kit. The purified DNA was eluted in 30 µl of Elution Buffer followed by a Real-time PCR assay performed with a 3 μl aliquot as template, primers (designed to amplify the ACTB gene), and Fast SYBR Green PCR Master Mix using the StepOnePlus™ Real-Time PCR system (Applied Biosystems). The results confirm the efficiency of DNA extraction from as low as 1 µl blood samples.

Genomic DNA Extraction Kits Specifications

 
SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300		GB100
GB300		GT050
GT100
GT300		GP100
GPM010
GPM020		GBB100/101
GBB300/301		GBYB100
GBYB300		GMB100
GMB300		GSK100
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg50 µg (Mini)
500 µg (Maxi)		50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
 20 mg animal tissue200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
 1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
 hair2x108 yeast/fungus    1-100 µl saliva 
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme Proteinase KN/AProteinase KProteinase K
Typical Yield5 µg from
200 µl blood		4-10 µg from
300 µl blood		10-40 µg from
20 mg tissue		3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
 10-40 µg from
20 mg tissue		  80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109 Bacillus subtilis   
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)		30-200 µl30-200 µl30-100 µl50-100 µl
Operation Time<20 minutes<20 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes

RELATED PRODUCTS

 
Product NameRXNSCatalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)100, 300GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)100, 300GB100/300
Genomic DNA Maxi Kit (Plant)10, 25GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)10, 25GDM10/25
Genomic DNA Mini Kit (Tissue)50, 100, 300GT050/100/300
Genomic DNA Mini Kit (Plant)100GP100
Geneius™ Micro gDNA Extraction Kit100GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit100GSK100/300
Presto™ Mini gDNA Yeast Kit100, 300GBYB100/300
Presto™ Mini gDNA Bacteria Kit100, 300GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit4 x 96, 10 x 9696GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit4 x 96, 10 x 9696GPP04/10
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