Blood/Cell DNA Maxi Kit (GDM010, GDM025)DNA Extraction/Genomic DNA Purification
The Genomic DNA Maxi Kit (Blood/Cultured Cell) is optimized for DNA purification (including genomic, mitochondrial and viral DNA) from up to 10 ml of frozen blood or fresh blood and up to 1 x 10⁸ cultured cells using an efficient maxiprep system. This DNA Extraction Kit uses Protease to reduce hemoglobin contamination while chaotropic salt and detergents are used to lyse cells and degrade protein, allowing the DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in 1 hour without phenol/chloroform extraction or alcohol precipitation. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.