旭基科技股份有限公司

Specifications (Cat. # GDM010, GDM025)

• High Yield: up to 200 μg from 10 ml of whole blood
• Large Sample Size: up to 10 ml of frozen blood or fresh blood, cultured cells (up to 1 x 10⁸)
• Genomic glass fiber maxi spin column optimized for genomic DNA purification
• Elution Volume: 500 µl ~ 2 ml
• Storage: dry at room temperature (15-25°C)
• Operation time: within 1 hour

APPLICATIONS

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

• GB Buffer
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
• Protease
• GD Maxi Columns
• Collection Tubes

QUALITY CONTROL

The quality of the Genomic DNA Maxi Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from a 10 ml of whole human blood is extracted using the Genomic DNA Maxi Kit (Blood/Cultured Cell). The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.

Genomic DNA Maxi Kit Functional Data

Figure 1 .Genomic DNA from 10 ml whole blood samples was extracted using the Genomic DNA Maxi Kit (Blood/Cultured Cell). 0.5 μl aliquots from 1 ml eluates of purified genomic DNA were analyzed by electrophoresis on a 0.8% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 

Sample

DNA Conc.

260/280

260/230

Yield

1

180.6 ng/µl

1.84

2.28

180.6 µg

2

195.8 ng/µl

1.86

2.15

195.8 µg

3

187.5 ng/µl

1.84

2.21

187.5 µg