Blood DNA Midi Kit (GDI100)DNA Extraction/Genomic DNA Purification
The Genomic DNA Midi Kit Blood is optimized for DNA purification (including genomic, mitochondrial and viral DNA) from up to 3 ml of fresh blood using an efficient mini spin column and column extension tube system. This DNA Extraction Kit uses Protease to reduce hemoglobin contamination while chaotropic salt and detergents are used to lyse cells and degrade protein, allowing the DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed 30 minutes without phenol/chloroform extraction or alcohol precipitation. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.