PRODUCTS | Geneaid Biotech Ltd

Yeast Genomic DNA Kit (GBYB100, GBYB300)

DNA Extraction/Genomic DNA Purification

The Presto™ Mini gDNA Yeast Kit was designed for rapid isolation of genomic DNA from cultured yeast and other fungus species. The yeast/fungus cells are directly added into beadbeating tubes containing ceramic beads and lysis buffer. Samples are efficiently lysed without the use of degestive enzymes (such as Lyticase or Zymolase) using a bead beating instrument or a standard vortex. The cell lysate is then mixed with Binding Buffer and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

DOCUMENTS

COMPLETE PROTOCOL

DESCRIPTION

Specifications (Cat. # GBYB100, GBYB300)

Purify genomic DNA from (up to 2 x 10⁸) yeast and other fungus species
Genomic DNA Yield: up to 10 µg of genomic DNA from 2 x 10⁸ Saccharomyces cerevisiae
Format: beadbeating tubes and spin columns
Elution volume: 30-200 µl
Time: 40 minutes
Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, restricion enzyme digestion and sequencing reactions

COMPONENTS

GT Buffer
PR Buffer
GB Buffer
W1 Buffer
Wash Buffer
Elution Buffer
RNase A (50 mg/m)
GD Columns
Beadbeating Tubes Type B
2 ml Collection Tubes

QUALITY CONTROL

The quality of the Presto™ Mini gDNA Yeast DNA Kit is tested on a lot-to-lot basis by isolating genomic DNA from 2 x10⁸ Saccharomyces cerevisiae cells. Following the purification process, greater than 8 μg of genomic DNA is obtained and the A260/A280 ratio is between 1.7-2.0. The purified genomic DNA is analyzed by electrophoresis.

Geneaid™ Yeast DNA Extraction Kit Funtional Test Data

Figure 1 .Genomic DNA was extracted using the Presto™ Mini gDNA Yeast DNA Extraction Kit. Saccharomyces cerevisiae (50 mg, 100 mg, 200 mg) was harvested by centrifugation at 5,000 x g for 10 minutes. 5 µl aliquots of purified genomic DNA from a 100 µl eluate were analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

Test

DNA Conc.

260/280

260/230

Yield

21.2 ng/µl

1.89

2.09

2.1 µg

22.2 ng/µl

1.90

2.05

2.2 µg

37.5 ng/µl

1.91

2.18

3.8 µg

40.7 ng/µl

1.90

2.05

4.1 µg

75.3 ng/µl

1.86

2.07

7.5 µg

84.9 ng/µl

1.87

2.11

8.5 µg

Genomic DNA Extraction Kits Specifications

Specifications	gSYNC™ DNA Extraction Kit	Genomic DNA Mini Kit Blood Cultured Cell	Genomic DNA Mini Kit Tissue	Genomic DNA Mini/Maxi Kit Plant	Presto™ Mini gDNA Bacteria Kit	Presto™ Mini gDNA Yeast Kit	Geneius™ Micro gDNA Extraction Kit	Presto™ Buccal Swab gDNA Extraction Kit
Cat. #	GS100 GS300	GB100 GB300	GT050 GT100 GT300	GP100 GPM010 GPM020	GBB100/101 GBB300/301	GBYB100 GBYB300	GMB100 GMB300	GSK100 GSK300
Format	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column
Binding Capacity	50 µg	50 µg	50 µg	50 µg (Mini) 500 µg (Maxi)	50 µg	50 µg	50 µg	50 µg
Sample	200 µl blood	300 µl blood	30 mg animal tissue	Mini: 100 mg plant tissue	1x10⁹ bacterial cells	2x10⁸ yeast cells	1-100 µl blood	buccal swabs
	20 mg animal tissue	200 µl buffy coat		Maxi: 1 g plant tissue	200 µl blood		FTA® Cards
	1x10⁷ cultured cells	1x10⁷ cultured cells			1 ml urine, plasma		1 ml urine
	hair	2x10⁸ yeast/fungus					1-100 µl saliva
Micropestle	N/A	N/A	Yes	N/A	N/A	N/A	N/A	N/A
Filter Column	N/A	N/A	N/A	Yes	N/A	N/A	N/A	Yes
Enzyme	Proteinase K	N/A	Proteinase K	N/A	Lysozyme Proteinase K	N/A	Proteinase K	Proteinase K
Typical Yield	5 µg from 200 µl blood	4-10 µg from 300 µl blood	10-40 µg from 20 mg tissue	3-5 µg from 100 mg Arabidopsis leaf (Mini)	37 µg from 1x10⁹ E. coli	10 µg from 2x10⁸ S. cerevisiae cells	300 ng from 6 mm blood spots	2 µg per swab
	10-40 µg from 20 mg tissue			80 µg from 500 mg Lemon leaf (Maxi)	10-15 µg from 1x10⁹ Bacillus subtilis
Elution Volume	30-100 µl	30-200 µl	30-200 µl	30-200 µl (Mini) 1 ml (Maxi)	30-200 µl	50-100 µl	30-100 µl	50-100 µl
Operation Time	<20 minutes	<20 minutes	30 minutes	<30 minutes	<40 minutes	<40 minutes	<20 minutes	<20 minutes

RELATED PRODUCTS

Product Name	RXNS	Catalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)	100, 300	GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)	100, 300	GB100/300
Genomic DNA Maxi Kit (Plant)	10, 25	GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)	10, 25	GDM10/25
Genomic DNA Mini Kit (Tissue)	50, 100, 300	GT050/100/300
Genomic DNA Mini Kit (Plant)	100	GP100
Geneius™ Micro gDNA Extraction Kit	100	GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit	100	GSK100/300
Presto™ Mini gDNA Yeast Kit	100, 300	GBYB100/300
Presto™ Mini gDNA Bacteria Kit	100, 300	GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GPP04/10

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