Bacteria Advanced DNA Kit (GBBA100, GBBA300)DNA Extraction/Genomic DNA Purification
The Presto™ gDNA Bacteria Advanced Kit is designed for rapid isolation of genomic DNA from cultured Gram (+) positive and Gram (-) negative bacteria. After directly adding into pre-filled beadbeating tubes containing zirconia/ceramic beads and lysis buffer, bacterial cells are efficiently lysed using a standard vortex or bead beating instrument. Enzymatic digestion with Lysozyme and Proteinase K is not required. The cell lysate is then mixed with a binding buffer, followed by bind, wash and elute using a spin column designed specifically for bacteria DNA extraction. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 30 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.