旭基科技股份有限公司

Specifications (Cat. # 96GPP04, 96GPP10)

• Purify 96 wells of pure genomic DNA from a variety of plant species in 60 minutes!
• Sample Volume: 100 mg of fresh plant tissue and 25 mg of dried plant tissue per well
• Yield: up to 30 μg of genomic DNA from 100 mg of fresh plant tissue per well
• Format: Presto™ 96 Well gDNA Binding Plates
• Elution Volume: 200~400 µl
• Centrifuge or vacuum manifold protocol
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

• GP1 Buffer and GPX1 Buffer
• GP2 Buffer
• GP3 Buffer
• RNase A
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• Presto™ gDNA 96 Well Binding Plates
• 96 Deep Well Plates
• Microtubes (Racked)
• Microtubes (8-strip)
• Caps for Microtubes (8-strip)

QUALITY CONTROL

The quality of the Presto™ 96 Well Plant Genomic DNA Extraction Kit is tested on a lot-to-lot basis by purifying genomic DNA from corn leaf samples. The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.

Presto™ 96 Well Plant Genomic DNA Extraction Kit Functional Test Data

Figure 1 .Genomic DNA was extracted from 50 mg of fresh corn leaf samples using the Presto™ 96 Well Plant Genomic DNA Extraction Kit. The purified genomic DNA was eluted in 200 µl of Elution Buffer and 15 µl aliquots of the final sample (chosen from 3 random wells) were analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid™ 1 Kb DNA Ladder

 

Sample

ng/µl

260/280

260/230

Yield

1. Corn Leaf

77.7

1.80

2.02

13.6 µg

2. Corn Leaf

78.8

1.80

2.05

13.8 µg

3. Corn Leaf

76

1.79

1.99

13.3 µg