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Gel Extraction Kit (DFG100/DFG300)

DNA Extraction/Gel Extraction

The GenepHlow™ Gel Extraction Kit was designed to recover or concentrate DNA fragments from agarose gel. This gel extraction kit includes QG Buffer premixed with a pH indicator to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # DFG100, DFG300)

  • High Efficiency: up to 90% recovery from agarose gels
  • Only 20 minutes gel extraction
  • Convenient: QG binding buffer is premixed with a pH indicator to allow for easy determination of optimal pH
  • Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high following gel dissociation
  • Sample Size: up to 300 mg of agarose gel slice
  • Broad Fragment Size Range: 70 bp–20 kb
  • Spin Columns: glass fiber membrane optimized for gel extraction
  • Elution Volume: 20-50 µl
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

COMPONENTS

  • QG Buffer (premixed with a pH indicator)
  • 3M Sodium Acetate (pH5.0)
  • W1 Buffer
  • Wash Bufffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • DFH Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the GenepHlow™ Gel Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from agarose gel and analyzed by electrophoresis.

GenepHlow™ Gel Extraction Kit Functional Test Data

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Figure 1 .Gel slice DNA fragments ranging from 250 bp-10 kb were extracted using the GenepHlow™ Gel Extraction Kit (lane 1,2,3). The purified DNA from a 50 µl eluate was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1100 ng)

 
Test
DNA Conc.
260/280
Yield
Recovery
1
18.2 ng/µl
1.84
910 ng
82%
2
18.7 ng/µl
1.82
935 ng
85%
3
19.2 ng/µl
1.82
960 ng
87.3%
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Figure 2.A pH indicator is premixed with the binding buffer to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow.

Gel Extraction and PCR Cleanup Kits Specifications

 
SpecificationsGenepHlow™ Gel Extraction KitGenepHlow™ PCR Cleanup KitGenepHlow™ Gel/PCR KitGenepHlow™ DNA Cleanup Maxi KitPresto™ 96 Well Gel Extraction KitSmall DNA Fragments Extraction KitLarge DNA Fragments Extraction Kit
Cat. #DFG100
DFG300		DFC100
DFC300		DFH100
DFH300		DFM010
DFM025		96DFG04
96DFG10		DF101
DF301		DFL100
DFL300
FormatSpin ColumnSpin ColumnSpin ColumnMaxi Spin Column96 Well PlateSpin ColumnSilica Matrix
Binding Capacity10 µg10 µg10 µg200 µg10 µg10 µg5 µg
SampleAgarose Gel SlicePCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/Restriction Enzyme Digest ProductAgarose Gel SliceAgarose Gel Slice/PCR ProductAgarose Gel Slice/PCR Product
Fragment Size70 bp-20 kb70 bp-20 kb70 bp-20 kb100 bp-10 kb70 bp-20 kb40-200 bp100 bp-50 kb
pH IndicatorYesYesYesYesN/AN/AYes
RecoveryUp to 90%Up to 95%Up to 95%Up to 80%Up to 80%Up to 95%Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Elution Volume20-50 µl20-50 µl20-50 µl500-1000 µl60-80 µl20-50 µl10-20 µl
Operation Time20 minutes10 minutes<20 minutes<25 minutes45 minutes<20 minutes<20 minutes

RELATED PRODUCTS

 
Product NameRXNSCatalogue Number
GenepHlow™ Gel Extraction Kit100/300DFG100/300
GenepHlow™ PCR Cleanup Kit100/300DFC100/300
GenepHlow™ Gel/PCR Kit100/300DFH100/300
GenepHlow™ DNA Cleanup Maxi Kit10/25DFM010/25
Presto™ 96 Well PCR Cleanup Kit4 x 96/10 x 9696DFH04/10
Small DNA Fragments Extraction Kit100/300DF101/301
Large DNA Fragments Extraction Kit100/300DFL100/300
Gel/PCR DNA Fragments Extraction Kit100/300DF100/300
Presto™ 96 Well Gel Extraction Kit4 x 96/10 x 9696DFG04/10
GenepHlow™ DNA Cleanup Midi Kit100/300DFI100/300
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