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Small DNA Fragments Kit (DF101, DF301)

DNA Extraction/Gel Extraction

The Small DNA Fragments Extraction Kit is a Gel Extraction Kit and PCR Cleanup Kit in 1 convenient system optimized to recover or concentrate 40-200 bp DNA fragments from agarose gel, PCR, or other enzymatic reactions. For processing 70 bp-10 kb DNA fragments, the Gel/PCR DNA Fragments Extraction Kit is also available. For processing 10-50 kb DNA fragments, the Large DNA Fragments Extraction Kit is also available. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are up to 90% for Gel Extraction and up to 95% for PCR Cleanup. The eluted DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # DF101, DF301)

  • Purify small DNA in as little as 10 minutes!
  • Up to 95% recovery of small DNA fragments
  • Optimized for 40 bp–200 bp DNA fragment recovery
  • Sample Size: up to 300 mg (agarose gel slice), up to 100 μl (PCR products)
  • Spin Columns: glass fiber membrane optimized for both gel extraction and PCR cleanup of small DNA fragments
  • Elution Volume: 20-50 µl
  • Storage: dry at room temperature (15-25ºC)

APPLICATIONS

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation

COMPONENTS

  • SD Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • SD Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Small DNA Fragments Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions and analyzed by electrophoresis.

Small DNA Fragments Extraction Kit Interactive Protocol

mall DNA Fragments Extraction Kit Functional Test Data

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Figure 1 .A 60 bp DNA fragment from Escherichia coli 16S ribosomal DNA (1 µg) was extracted from a PCR product using the Small DNA Fragments Extraction Kit. The recovered DNA concentration was determined by spectrophotometer and analyzed by electrophoresis on a 1.5% agarose gel. Lane 1: Unpurified 60 bp DNA fragment (1 µg), Lane 2: Purified 60 bp DNA fragment (0.71 µg), M = Geneaid 100 bp DNA Ladder

 
DNA Conc.
260/280
Elution Volume
Total yield
Recovery
14.20 μg/ml
1.73
50 µl
0.71 μg
71%
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Figure 2 .A 60 bp DNA fragment from Escherichia coli 16S ribosomal DNA (1 µg) was extracted from gel using the Small DNA Fragments Extraction Kit. The recovered DNA concentration was determined by spectrophotometer and analyzed by electrophoresis on a 1.5% agarose gel. Lane 1: Unpurified 60 bp DNA fragment (1 µg), Lane 2: Purified 60 bp DNA fragment (0.60 µg), M = Geneaid 100 bp DNA Ladder

 
DNA Conc.
260/280
Elution Volume
Total yield
Recovery
12.00 μg/ml
1.63
50 µl
0.60 μg
60%

Gel Extraction and PCR Cleanup Kits Specifications

 
SpecificationsGenepHlow™ Gel Extraction KitGenepHlow™ PCR Cleanup KitGenepHlow™ Gel/PCR KitGenepHlow™ DNA Cleanup Maxi KitPresto™ 96 Well Gel Extraction KitSmall DNA Fragments Extraction KitLarge DNA Fragments Extraction Kit
Cat. #DFG100
DFG300		DFC100
DFC300		DFH100
DFH300		DFM010
DFM025		96DFG04
96DFG10		DF101
DF301		DFL100
DFL300
FormatSpin ColumnSpin ColumnSpin ColumnMaxi Spin Column96 Well PlateSpin ColumnSilica Matrix
Binding Capacity10 µg10 µg10 µg200 µg10 µg10 µg5 µg
SampleAgarose Gel SlicePCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/Restriction Enzyme Digest ProductAgarose Gel SliceAgarose Gel Slice/PCR ProductAgarose Gel Slice/PCR Product
Fragment Size70 bp-20 kb70 bp-20 kb70 bp-20 kb100 bp-10 kb70 bp-20 kb40-200 bp100 bp-50 kb
pH IndicatorYesYesYesYesN/AN/AYes
RecoveryUp to 90%Up to 95%Up to 95%Up to 80%Up to 80%Up to 95%Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Elution Volume20-50 µl20-50 µl20-50 µl500-1000 µl60-80 µl20-50 µl10-20 µl
Operation Time20 minutes10 minutes<20 minutes<25 minutes45 minutes<20 minutes<20 minutes

RELATED PRODUCTS

 
Product NameRXNSCatalogue Number
GenepHlow™ Gel Extraction Kit100/300DFG100/300
GenepHlow™ PCR Cleanup Kit100/300DFC100/300
GenepHlow™ Gel/PCR Kit100/300DFH100/300
GenepHlow™ DNA Cleanup Maxi Kit10/25DFM010/25
Presto™ 96 Well PCR Cleanup Kit4 x 96/10 x 9696DFH04/10
Small DNA Fragments Extraction Kit100/300DF101/301
Large DNA Fragments Extraction Kit100/300DFL100/300
Gel/PCR DNA Fragments Extraction Kit100/300DF100/300
Presto™ 96 Well Gel Extraction Kit4 x 96/10 x 9696DFG04/10
GenepHlow™ DNA Cleanup Midi Kit100/300DFI100/300
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