旭基科技股份有限公司

Advantages (Cat. # GZR050, GZR100, GZR200)

• Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
• Sample Volume: up to 300 μl of blood, buffy coat, serum, plasma, up to 5 x 10⁶ cultured cells, 50-100 mg of tissue
• A cost effective, scalable solution
• Format: phenol, chlorform, guanidine isothiocyanate
• Storage: dry at 2ºC to 25ºC

APPLICATIONS

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

COMPONENTS

• GENEzol™ Reagent

QUALITY CONTROL

GENEzol™ Reagent is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. RNA from a 1 ml human blood sample is extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.

GENEzol™ Reagent Comparison Data

Figure 1 .Bacterial RNA was extracted from 5 x 10⁸ E. coli cells using our top competitor's TRI reagent and GENEzol™ Reagent. The E. coli cells were incubated with lysozyme for 5 minutes at room temperature. 1 ml of reagent was then added to the cell lysate. The RNA pellets were dissolved in 50 μl of RNase-free water.

TRI Reagent = 1, 2
GENEzol™ Reagent = 3, 4

 

Sample

ng/µl

260/280

260/230

Yield

1

241.3

1.77

0.66

12.1

2

260.2

1.83

0.85

13.0

3

315.6

1.78

0.75

15.8

4

293.4

1.81

0.80

14.7

GENEzol™ Reagent Functional Test Data

Figure 2 .RNA from a 1 ml human blood sample was extracted using GENEzol™ Reagent. 10 µl from a 50 µl eluate of RNA was analyzed by electrophoresis on a 0.8% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 

Sample

RNA Conc.

260/280

Yield

1

119.9 µg/ml

1.82

6.0 µg

2

135.5 µg/ml

1.76

6.8 µg

3

176.1 µg/ml

1.77

8.8 µg

GENEzol™ Reagent real-time PCR Data

RNA was extracted from 200 µl of human blood (5.2 x 10¹⁰ copy/ml) using GENEzol™ TriRNA Pure Kit (red line).
RNA standard (10⁷ copy/ml) (light green line)
RNA standard (10⁶ copy/ml) (dark green line)
RNA standard (10⁵ copy/ml) (light blue line)
RNA standard (10⁴ copy/ml) (dark blue line)

Figure 3 .Quantitative analysis of human beta globin mRNA extracted by GENEzol™ using Taqman probe 1-step qRT-PCR assay. The assay was run on a BioRad IQ5 thermal cycler.