旭基科技股份有限公司

Close
SEARCH
*

HealthTrace™ Dye as Loading Buffer, 6x (HTLD001s, HTLD001, HTLD005)

Others/DNA Ladders

HealthTrace™ Dye as Loading Buffer, 6x is an eco-friendly DNA visualization dye designed to replace the use of hazardous ethidium bromide in DNA gels and running buffer. Packaged as Geneaid DNA Loading Dye (6X), this non-toxic and non-mutagenic dye features two tracking dyes migrating at 4,000 bp and 400 bp. DNA samples, when mixed with HealthTrace™ Dye as Loading Buffer, can be seamlessly applied to both agarose and polyacrylamide gels. Upon application, HealthTrace™ Dye swiftly forms a complex with the DNA, co-migrating during electrophoresis. Emitting green fluorescence upon binding to DNA, immediate visualization of samples is facilitated post-electrophoresis using standard UV or blue light illumination for gel documentation. There is no requirement for subsequent post-staining or destaining procedures.

DOCUMENTS

DESCRIPTION

Advantages (Cat. # HTLD001s, HTLD001, HTLD005)

  • Loading DNA samples into gel electrophoresis wells
  • Easy visual tracking of DNA migration during electrophoresis
  • Instant results
  • Eliminates Hazardous Ethidium Bromide Use
  • High sensitivity: as low as 6 ng of DNA can be detected
  • Dry Ice is not required for shipping

STORAGE

2-8 ºC for up to 12 months 

COMPONENTS

  • HealthTrace™ Dye (Excitation: 268, 491 nm, Emission: 530 nm)
  • bromophenol blue
  • xylene cyanol FF
  • glycerol

QUALITY CONTROL

The quality of the HealthTrace™ Dye as Loading Buffer, 6x is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system.

HealthTrace™ Dye as Loading Buffer, 6x Test Data 

*






Figure 1.Electrophoresis of the HealthTrace™ Dye as Loading Buffer. The HealthTrace™ Dye was loaded on a 1% TAE agarose gel.  Digital camera photograph shows the position of the two tracking dye colors.

 

 
*

Figure 2. Sensitivity of HealthTrace™ Dye. HealthTrace™ Dye as Loading Buffer, 6x can detect 6 ng DNA for a 500 bp fragment and 12 ng DNA for a 100 bp fragment.

 

 

RELATED PRODUCTS

 
Go-top