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Figure 1.The 50 bp DNA ladder was used to simulate PCR amplicons of varying fragment sizes (50-1,500bp, 1000 ng, 20 µL). Purification was performed using Geneaid Presto™ PureXP Magbeads and B brand Magbeads, respectively (in triplicates). The purified products were quantified using a Qubit (Thermo) and analyzed by capillary electrophoresis. The results demonstrated that the Presto™ PureXP Magbeads exhibited a higher recovery rate (84.6%) compared to the B brand (78.6%). Capillary electrophoresis also showed consistent results.
Figure 2.Sheared DNA (green line) was purified separately using Geneaid Presto™ PureXP Magbeads and B brand Magbeads. After adjusting the concentrations of the purified products to the same level, capillary electrophoresis analysis was performed. The results show that the two curves representing Geneaid Presto™ PureXP Magbeads (red line) and Brand B Magbeads (blue line) completely overlap, confirming that the different DNA fragment sizes recovery profile of Geneaid Presto™ PureXP Magbeads is identical to that of B Brand Magbeads.

Figure 3.Sheared DNA was recovered using Geneaid Presto™ PureXP Magbeads with different volume ratios to remove various DNA fragment sizes. After adjusting the concentrations of the purified products to the same level, capillary electrophoresis analysis was performed. The results showed that a Magbead:DNA ratio of 1.8x effectively removed fragments smaller than 100 bp, a ratio of 0.9x removed fragments smaller than 200 bp, 0.7x removed fragments smaller than 250 bp, and 0.6x removed fragments smaller than 300 bp. Geneaid Presto™ PureXP Magbeads demonstrated optimal size selection functionality.