旭基科技股份有限公司

Specifications (Cat. # DP100, DP300)

• Recover up to 90% of DNA products within 10 minutes!
• Sample Volume: up to 100 μl of DNA products (plasmid BAC/PAC, genomic, mitochondrial, viral, bacteriophage etc.) which were previously isolated using a variety of isolation methods as well as from enzymatic reactions, Proteinase K digestion or other contaminated preparations
• Spin Columns: glass fiber membrane optimized for purifying DNA products
• Broad Fragment Size Range: 70 bp–20 kb
• Elution Volume: 20-50 µl
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS

PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling, Ligation

COMPONENTS

• DNA Pure Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
• DP Columns
• 2 ml Collection Tubes

QUALITY CONTROL

The quality of the DNA Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA of various sizes are purified from aqueous solutions and analyzed by electrophoresis.

DNA Pure Kit Funtional Test Data

Figure 1 .4 µg genomic DNA samples were extracted from whole human blood and purified using the DNA Cleanup Kit. 10 µl of purified DNA from a 100 µl eluates was analyzed by electrophoresis on a 0.8% agarose gel. 1 = Unpurified 4 µg genomic DNA sample, 2-4 = Purified samples,

M = Geneaid 1 Kb DNA Ladder

 

Sample

Yield

260/280

Recovery

1

3.10 µg

1.83

72 %

2

2.80 µg

1.80

72 %

3

2.70 µg

1.81

72 %